Monoclonal Anti-Collagen, Type IV antibody produced in mouse
SIGMA/C1926 - clone COL-94, ascites fluid
Synonym: Anti-Collagen IV; Collagen IV Detection Antibody; Mouse Anti-Collagen IV
MDL Number: MFCD00162240
Product Type: Chemical
Immunohistochemistry Staining of formalin-fixed, paraffin-embedded human tongue sections with 1:500 Monoclonal Anti-Collagen, Type IV, Clone: COL-94 (Cat. No. C1926) using biotin/ExtrAvidin®-Peroxidase.
Immunohistochemistry Formalin-fixed, paraffin-embedded tissue section of human tongue stained with 1:500 Monoclonal Anti-Collagen, Type IV antibody produced in Mouse clone: COL-94 (Cat. No. C1926) followed by Biotin (Cat. No. B7151) and by ExtrAvidin®-Peroxidase (Cat. No. E2886).
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | COL-94, monoclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| isotype | IgG1 |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | dot blot: suitable |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:500 using protease-digested sections of human tissue | |
| immunohistochemistry (frozen sections): suitable | |
| indirect ELISA: suitable | |
| microarray: suitable | |
| UniProt accession no. | P53420  |
| Application: | Monoclonal Anti-Collagen, Type IV antibody produced in mouse has been used in • indirect immunolabeling • immunofluorescence staining • western blotting • immunohistochemistry • immunocytochemistry |
| Biochem/physiol Actions: | Collagens are known to have multiple functions. They are involved in tissue differentiation, morphogenesis and repair; play a role in the maintenance of tissue architecture; and form the filtration barrier of blood vessels. |
| Biochem/physiol Actions: | Using immunohistochemical staining of basal laminae, the antibody recognizes an epitope located on the α1 and/or α2 chains of human collagen type IV. The antibody shows reactivity with native primate collagen type IV in ELISA and dot blot assay. Using the dot blot technique, the antibody shows no cross-reactivity with human collagen types I, II, III, V, VI and VII, human vitronectin, fibronectin and chondroitin sulfate types A, B and C. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-Collagen Type IV (mouse IgG1 isotype) is derived from the COL-94 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with human collagen Type IV. |
| General description: | The composition of the extracellular matrix of all vertebrates is dominated by a class of molecules known as collagens, each with a unique feature suited to its function and location. Type IV collagen is a major constituent of basement membranes. This molecule is a continuous triple helix, about 400 nm in length, composed of at least two different α chain subunits termed α1 (IV) and α2 (IV) which may be composed of the same or different molecules. Upon proteolysis, fragments of 125 kDa, 95 kDa, and 60 kDa are released from a parent α1 (IV) chain, whereas fragments of 115 kDa, 80 kDa, and 45 kDa apparently originate from a distinct α2 (IV) chain. |
| Immunogen: | human collagen type IV |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |