ColorBurst™ Electrophoresis Marker
SIGMA/C1992 - mol wt 8,000-220,000 Da
Synonym: protein markers; protein molecular weight markers; protein standards
MDL Number: MFCD03456450
Product Type: Chemical
ColorBurst (Cat. No. C1992): Bands transferred to nitrocellulose membranes. Transfers were completed in 90 minutes at 70 volts with Towbin’s buffer (Tris-Glycine in 20% methanol). Molecular Masses (kDa) given are apparent values as compared to Sigma’s wide range marker set (Cat. No. S8445). The ColorBurst markers migrate differently with respect to Cat. No. S8445 in the different gel systems tested.
| form | solution |
| mol wt | 8,000-220,000 Da |
| pH | 8 |
| Quality Level | 200  |
| shipped in | wet ice |
| storage temp. | −20°C |
| Application: | Brilliantly colored, exceptionally well-resolved, convenient, and stable, ColorBurst protein molecular weight markers perform impressively in a variety of gel compositions and concentrations. ColorBurst Markers are composed of 8 polypeptides which have been chemically reduced and conjugated to brilliantly colored dyes. Conveniently, ColorBurst Markers require no resuspension, reduction, or heating prior to use. |
| Application: | Colorburst can be used to estimate sample molecular weights, to monitor the progress of an electrophoretic run, or to confirm that an electroblot is complete. |
| Legal Information: | ColorBurst is a trademark of Sigma-Aldrich Co. LLC |
| Other Notes: | Solution contains myosin-violet, BSA-red, GDH-blue, ADH-red, Carbonic Anhydrase-orange, Trypsin Inhibitor-blue, Lysozyme-red, Aprotinin-blue, 200 mM Tris, SDS and, formamide. Each lot provides the apparent molecular weights determined by comparison to an unconjugated standard. |
| Preparation Note: | Each vial of ColorBurst contains 500 μL of solution, enough for at least 50 mini gel applications. |
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H351 - H360D - H373 |
| Precautionary statements | P202 - P260 - P280 - P308 + P313 - P405 - P501 |
| Hazard Codes | T |
| Risk Statements | 61-40-48/22 |
| Safety Statements | 53-36/37-45 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Storage Temp. | −20°C |
| UNSPSC | 41105337 |