Concanavalin A from Canavalia ensiformis (Jack bean)

SIGMA/C2010 - Type IV, lyophilized powder

Synonym: Con A

CAS Number: 11028-71-0
EC Number: 234-258-2
MDL Number: MFCD00071069
Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-C2010-25MG	25 mg	$40.00 1/EA	Add To Favorites
45-C2010-100MG	100 mg	$98.80 1/EA	Add To Favorites
45-C2010-250MG	250 mg	$205.00 1/EA	Add To Favorites
45-C2010-1G	1 g	$479.00 1/EA	Add To Favorites

This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: C2010-100MG

Properties
Descriptions
Safety Info.
Basic Data

form	lyophilized powder
impurities	Carbohydrate, essentially free
	salt, essentially free
potency	<64 μg per mL agglutination activity
Quality Level	300
storage temp.	−20°C
type	Type IV

Analysis Note:	Highly purified by specific affinity chromatography
Analysis Note:	Where reported, agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, containing Ca²⁺ and Mn²⁺ of a 1 mg per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes after 1 hr incubation at 25 °C.
Application:	Concanavalin A from Canavalia ensiformis (Jack bean) has been used: • as T-lymphocyte mitogen in lymphocyte proliferation assay for correlations with organochlorine contaminants (OCs) • for in vitro stimulation of T cell hybridomas, BW5147 cells and primary CD4⁺ T cells for T cell vaccination and for induction of hepatitis • for the stimulation of mice splenocytes in the suppression of T-lymphocytes proliferation assay • for the stimulation of spleen in mitogen assays
Biochem/physiol Actions:	Con A is not blood group specific but has an affinity for terminal α-D-mannosyl and α-D-glucosyl residues. Ca²⁺ and Mn²⁺ ions are required for activity. Con A dissociates into dimers at pH 5.6 or below. Between pH 5.8 and pH 7.0, Con A exists as a tetramer; above pH 7.0 higher aggregates are formed. Con A exhibits mitogenic activity which is dependent on its degree of aggregation. Succinylation results in an active dimeric form which remains a dimer above pH 5.6.
General description:	Concanavalin A (con A) is a plant lectin refined from jack beans. It is a tetrameric protein, which has four identical 28 kDa subunits. Con A has the ability to connect with the mannose residues of several glycoproteins and can stimulate lymphocytes.
Packaging:	1 g in glass bottle
Packaging:	100, 250 mg in glass bottle
Packaging:	25 mg in poly bottle

Symbol	GHS08
Signal word	Danger
Hazard statements	H317 - H334 - H361
Precautionary statements	P201 - P202 - P280 - P302 + P352 - P304 + P340 + P312 - P308 + P313
Hazard Codes	Xn
Risk Statements	63-42/43
Safety Statements	24/25-36
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Storage Temp.	−20°C
UNSPSC	23151817

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