Cytochrome c from bovine heart
SIGMA/C2037 - ≥95% based on Mol. Wt. 12,327 basis
CAS Number: 9007-43-6
EC Number: 232-700-9
MDL Number: MFCD00130890
Product Type: Chemical
The electron transport protein complex of the inner mitochondrial membrane
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: C2037-5G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: C2037-100MG
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: C2037-250MG
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: C2037-500MG
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: C2037-50MG
| application(s) | cell analysis |
| assay | ≥95% based on Mol. Wt. 12,327 basis |
| biological source | bovine heart |
| form | powder or crystals |
| mol wt | 12327 Da |
| Quality Level | 300  |
| storage temp. | −20°C |
| suitability | suitable for molecular biology |
| technique(s) | cell based assay: suitable |
| UniProt accession no. | P62894  |
| Application: | Cytochrome c from bovine heart has been used:• for cytochrome oxidase staining using the mice brain tissue • as a component of control mixture to evaluate the accuracy of tandem mass spectra in identifying peptides • to study its effect on the stability, transport, and retention of biochar colloids (BCs) in saturated porous media under various solution pH and ionic strength (IS) conditions • as a component of staining solution for the detection of cytochrome oxidase activity in the brain tissue sections of mouse • as a component in the assay to study its superoxide scavenging efficiency • as a component of serum-free DMEM media to evaluate non-specific cytochrome C reduction in astrocytes and HT-29 cells |
| Biochem/physiol Actions: | Cytochrome C is a key mediator of oxidative phosphorylation. It mediates the electron transport between complex III and complex IV of the respiratory chain. Cytochrome c has been identified as an important mediator in apoptotic pathways. The release of mitochondrial cytochrome c into the cytoplasm stimulates apoptosis and is commonly used as an indicator of the apoptotic process in the cell. Investigation on the effect of Paris Saponin I (PS I) on human gastric carcinoma cell growth (SGC7901 cells) have shown an elevated level cytoplasmic cytochrome c. Results are an inhibition of proliferation in SGC7901 cells by inducing mitochondria-dependent apoptosis through cytochrome. |
| Biochem/physiol Actions: | The ready fluctuation of cytochrome c within the cell between ferrous and ferric states, makes it an efficient biological electron-transporter. It plays a vital role in cellular oxidations in both plants and animals. Generally regarded as a universal catalyst of respiration, it forms the essential electron-bridge between the respirable substrates and oxygen. |
| Biochem/physiol Actions: | The ready interconversion of cytochrome c between ferrous and ferric states makes it an efficient biological electron carrier. It plays a vital role in cellular oxidations in both plants and animals. Generally regarded as a universal link in the respiratory chain, it forms the essential electron-bridge between the respirable substrates and oxygen. |
| General description: | Cytochrome C is a heme protein. It is localized between the inner and outer mitochondrial membranes. |
| Other Notes: | View more information on cytochrome c and electron transport at www.sigma-aldrich.com/enz. |
| Packaging: | 1, 5 g in glass bottle |
| Packaging: | 50, 100, 500 mg in glass bottle |
| Preparation Note: | Prepared using TCA. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥95% based on Mol. Wt. 12,327 basis |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |