Anti-α-Catenin antibody produced in rabbit
SIGMA/C2081 - whole antiserum
Synonym: Anti-α-Catenin; Alpha Catenin Antibody; Alpha Catenin Antibody - Anti-α-Catenin antibody produced in rabbit
Product Type: Chemical
Western Blotting JOURNAL CITATION: Differential regulation of growth-promoting signalling pathways by E-cadherin. By: Georgopoulos, N. T., Kirkwood, L. A., et al. in PLoS One, 2010. PubMed ID: 21049033 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pone.0013621), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunocytochemistry-immunoflourescence JOURNAL CITATION: Differential regulation of growth-promoting signalling pathways by E-cadherin. By: Georgopoulos, N. T., Kirkwood, L. A., et al. in PLoS One, 2010. PubMed ID: 21049033 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pone.0013621), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Western Blotting JOURNAL CITATION: p120ctn and P-cadherin but not E-cadherin regulate cell motility and invasion of DU145 prostate cancer cells. By: Kümper, S. & Ridley, A. J. in PLoS One, 2010. PubMed ID: 20668551 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pone.0011801), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunohistochemistry Formalin-fixed, paraffin-embedded heat retrieval tissue section of bovine kidney stained with 1:2,000 Anti-α-Catenin antibody produced in rabbit (Cat. No. C2081) followed by Anti-Rabbit IgG (whole molecule)-Biotin antibody produced in goat (Cat. No. B8895) and by ExtrAvidin®-Peroxidase (Cat. No. E2886).
Immunofluorescence MDBK bovine kidney cells were fixed and permeabilized with Methanol followed by Acetone. Fixed cells were stained with 1:2,000 Anti-α-Catenin antibody produced in rabbit (Cat. No. C2081). The antibody was developed using Anti-Rabbit IgG (whole molecule)-FITC antibody produced in goat (Cat. No F9887). Cells were counterstained with DAPI (blue) to stain nuclei.
Immunoblotting Madin Darby Bovine Kidney (MDBK) extract was separated on SDS-PAGE and probed with AB To Catenin Alpha (RB) (Cat. No. C2081). The antibody was developed with Alk Phos APA Rabbit IgG (gt) (Cat. No. A9919) and a NBT/BCIP substrate. Antibody dilution 1/8,000
| antibody form | whole antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| mol wt | antigen ~102 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | several mammalian species |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | dot blot: suitable using α-catenin peptide amino acids 890-901 conjugated to BSA |
| immunohistochemistry (frozen sections): 1:2,000 using bovine kidney sections | |
| indirect immunofluorescence: 1:2,000 using cultured MDBK cells | |
| microarray: suitable | |
| western blot: 1:4,000 using cultured MDBK cells extract |
| Application: | Anti-α-Catenin antibody has been used: • in dot blot immunoassay • in immunoblotting • in immunofluorescence • in western blotting • in immunoprecipitation • in immunofluorescence staining • in coimmunoprecipitation |
| Biochem/physiol Actions: | Catenins bind, directly or indirectly, to the conserved cytoplasmic tail domain of the cell-adhesion cadherins. Cadherins are transmembrane cell surface glycoprotein molecules that mediate calcium-dependent intercellular interactions and are important for tissue morphogenesis. Catenins link E-cadherin to other integral membrane proteins such as Na+ /K+ -ATPase, or to cytoplasmic proteins such as fodrin, ankyrin, sarcoma (Src) and Yes kinases and are modulated by Wnt-1 protooncogene. They are considered good candidates for mediating transduction of cell-cell contact positional signals to the cell interior. α-Catenin appears to be capable of interacting with N-cadherin and P-cadherin. Absence of α-catenin is found in certain tumor cell lines. Frequent reduction of α-catenin levels in human carcinomas of the esophagus, stomach and colon is also reported. Prostate cancer development appears to be correlated with α-catenin gene deletions. |
| Biochem/physiol Actions: | Does not cross-react with β-catenin or γ-catenin (plakoglobin). |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Catenin are distinct peripheral cytosolic proteins, α, β, and γ -catenin (102 kDa, 94 kDa and 86 kDa, respectively) are found in varying abundance in many developing and adult tissues. Within its conserved regions, α-catenin shows 30% identity to vinculin, a protein found mainly in focal cell-cell and cell substrate adhesions. |
| Immunogen: | synthetic peptide corresponding to the C-terminal region (amino acids 890-901) of human/mouse α-catenin conjugated to KLH. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |