Anti-β-Catenin antibody produced in rabbit
SIGMA/C2206 - whole antiserum
Synonym: Anti-CTNNB; Anti-EVR7; Anti-MRD19; Anti-NEDSDV; Anti-armadillo
MDL Number: MFCD02685970
Product Type: Chemical
Immunohistochemistry JOURNAL CITATION: V600EBraf induces gastrointestinal crypt senescence and promotes tumour progression through enhanced CpG methylation of p16INK4a. By: Carragher, L. A., Snell, K. R., et al. in EMBO Mol Med, 2010. PubMed ID: 20941790 Image collected and cropped by CiteAb from the following publication, (http://embomolmed.embopress.org/cgi/doi/10.1002/emmm.201000099), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Western Blotting JOURNAL CITATION: p120ctn and P-cadherin but not E-cadherin regulate cell motility and invasion of DU145 prostate cancer cells. By: Kümper, S. & Ridley, A. J. in PLoS One, 2010. PubMed ID: 20668551 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pone.0011801), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunohistochemistry JOURNAL CITATION: V600EBraf induces gastrointestinal crypt senescence and promotes tumour progression through enhanced CpG methylation of p16INK4a. By: Carragher, L. A., Snell, K. R., et al. in EMBO Mol Med, 2010. PubMed ID: 20941790 Image collected and cropped by CiteAb from the following publication, (http://embomolmed.embopress.org/cgi/doi/10.1002/emmm.201000099), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunohistochemistry Formalin-fixed, paraffin-embedded heat retrieval tissue section of rat kidney stained with 1:2,000 Anti-β-Catenin antibody produced in Rabbit (Cat. No. C2206) followed by Biotin (Cat. No. B8895) and by ExtrAvidin®-Peroxidase (Cat. No. E2886).
Immunohistochemistry Formalin fixed, paraffin-embedded Rat kidney tissue sections were stained with 1:2,000 Anti-β-Catenin antibody produced in Rabbit (Cat. No. C2206), followed by 20 μg/mL Anti-Rabbit IgG (whole molecule)-Biotin antibody produced in Goat (Cat. No. B8895) and by 40 μg/mL ExtrAvidin®-Peroxidase (Cat. No. E2886).
Immunofluorescence MDBK bovine kidney cells were fixed and permeabilized with Methanol followed by Acetone. Fixed cells were stained with 1:2,000 Anti-β-Catenin antibody produced in Rabbit (Cat. No. C2206). The antibody was developed using Anti-Rabbit IgG (whole molecule)-FITC antibody produced in Goat (Cat. No. F9887). Cells were counterstained with DAPI (blue) to stain nuclei.
Immunoblotting Madin Darby Bovine kidney (MDBK) cells were separated on SDS-PAGE and probed with AB to Catenin Beta (RB) (Cat. No. C2206). The antibody was developed with Alkaline Phosphatase conjugate (Cat. No. A9919) and a NBT/BCIP substrate. Antibody dilution 1/8,000
| antibody form | whole antiserum |
| antibody product type | primary antibodies |
| application(s) | research pathology |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| mol wt | antigen 88-94 kDa |
| packaging | antibody small pack of 25 μL |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | several mammalian species |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | dot blot: suitable using the immunogen and related peptides conjugated to BSA |
| immunocytochemistry: 1:2,000 using cultured MDBK cells | |
| immunohistochemistry: 1:2,000 using bovine kidney frozen sections | |
| western blot: 1:4,000 using cultured MDBK cells |
| Application: | Anti-β-Catenin antibody produced in rabbit has been used in • co-immunoprecipitation • immunoblotting • immunofluorescence • immunoperoxidase assay |
| Biochem/physiol Actions: | Catenins bind, directly or indirectly, to the conserved cytoplasmic tail domain of the cell adhesion cadherins. The protein, β-catenin, binds directly to the cytoplasmic tail of E-cadherin. Plakoglobin (probably identical to γ-catenin) and β-catenin are structural and possibly functional mammalian homologues of armadillo (arm), a Drosophila protein involved in signal transduction. It binds to the amino terminus of α-catenin and also interacts with the cytosolic protein product of the human tumor suppressor gene Adenomatous polyposis coli (APC). The central core region of β-catenin is involved in mediation of the interaction of cadherin-catenin complex with the epidermal growth factor receptor. The protein, β-catenin, is the target of two signal transduction pathways mediated by the protooncogenes Src and wingless-related integration site (Wnt-1). p120cas which exhibits structural similarity to β-catenin and plakoglobin may represent another catenin associated with cadherin. |
| Biochem/physiol Actions: | The antibody does not cross react with α-catenin or γ-catenin (plakoglobin). |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Catenin are distinct peripheral cytosolic proteins and are classified into α-, β- and γ-catenin (102 kDa, 94 kDa and 86 kDa respectively. It is found in varying abundance in many developing and adult tissues. |
| Immunogen: | synthetic peptide corresponding to the C-terminal region (amino acids 768-781) of human/mouse β-catenin, conjugated with gluteraldehyde to KLH. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |