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Z-Gly-Pro-Arg p-nitroanilide acetate salt

SIGMA/C2276 - protease substrate

CAS Number: 66648-35-9 (free base)
Empirical Formula (Hill Notation): C27H34N8O7 · xC2H4O2
Molecular Weight: 582.61 (free base basis)
MDL Number: MFCD18782526
Linear Formula: C27H34N8O7 · xC2H4O2
Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-C2276-25MG 25 mg
 $369.00
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assay ≥98% (TLC)
form powder
InChI 1S/C27H34N8O7.C2H4O2/c28-26(29)30-14-4-8-21(24(37)32-19-10-12-20(13-11-19)35(40)41)33-25(38)22-9-5-15-34(22)23(36)16-31-27(39)42-17-18-6-2-1-3-7-18;1-2(3)4/h1-3,6-7,10-13,21-22H,4-5,8-9,14-17H2,(H,31,39)(H,32,37)(H,33,38)(H4,28,29,30);1H3,(H,3,4)/t21-,22-;/m0./s1
InChI key FPMAXHHYPVIJMC-VROPFNGYSA-N
Quality Level 200 
SMILES string CC(O)=O.NC(=N)NCCC[C@H](NC(=O)[C@@H]1CCCN1C(=O)CNC(=O)OCc2ccccc2)C(=O)Nc3ccc(cc3)[N+]([O-])=O
solubility methanol: 20 mg/mL, clear, colorless to light yellow
storage temp. −20°C
Application: Z-Gly-Pro-Arg p-nitroanilide acetate salt has been used as a substrate in prostate-specific antigen (PSA) activation protease assay for determining enterokinase-PSA-trypsin (EK-PSA-TRP) activity and to perform kinetic experiments. It has also been used as a substrate to measure the pancreatic trypsin activity chromogenically.
RIDADR NONH for all modes of transport
WGK Germany WGK 3
Flash Point(F) Not applicable
Flash Point(C) Not applicable
Purity ≥98% (TLC)
Storage Temp. −20°C
UNSPSC 12352204

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