Anti-K+/Cl- Cotransporter (KCC2) antibody produced in rabbit
SIGMA/C2366 - IgG fraction of antiserum, buffered aqueous solution
Synonym: Anti-DEE34; Anti-EIEE34; Anti-EIG14; Anti-KCC2; Anti-hKCC2
MDL Number: MFCD06798467
Product Type: Chemical
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| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | canine, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | western blot: 0.5-2.0 μg/mL using rat brain membrane preparation |
| UniProt accession no. | Q63633  |
| Application: | Anti-K+/Cl- Cotransporter (KCC2) specifically recognizes K+/Cl- cotransporter in rat brain membrane preparations by immunoblotting and Madin Darby Canine Kidney (MDCK) cells by immunocytochemistry. |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Immunocytochemistry (1 paper) |
| Biochem/physiol Actions: | Anti-K+/Cl- Cotransporter (KCC2) specifically recognizes K+/Cl- cotransporter. Wide range of species cross-reactivity expected due to sequence homology. |
| Biochem/physiol Actions: | Wide range of species cross-reactivity expected due to sequence homology. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Anti-K+/Cl- Cotransporter (KCC2) specifically recognizes K+/Cl- cotransporter (KCC2). The cation-chloride cotransporters (CCCs) are glycoproteins involved in transport of ions, includingchloride, across the ce |
| General description: | Transporters and exchangers play a critical role in the generation and dissipation of action potentials in nerve cells and in the maintenance of normal cell volume. They are also involved in a variety of mechanisms pertaining to the control of neuronal growth, maturation, synaptic plasticity and neuroendocrine functions The cation-chloride cotransporters (CCCs) are glycoproteins involved in transport of ions, including chloride, across the cell membrane without an accompanying net charge movement; thus this type of transport is driven without the direct hydrolysis of ATP. The energy for the transport is derived from the cation gradient generated by the Na+/K+/ATPase. The CCCs also play a critical role in influencing GABA- and glycine-mediated signaling. Other transporters that participate in chloride homeostasis include Na+-dependent and Na+-independent anion exchangers, which exchange chloride for HCO3- ions Three cotransporters belonging to this family include sodiumchloride( Na+/Cl-/ ) cotransporters (NCCs), sodiumpotassium-chloride (Na+/K+/2Cl-) cotransporters (NKCCs) and potassium-chloride (K+/Cl-) cotransporters (KCCs). K+/Cl- cotransporter (KCC2) is the major neuronal chloride transporter. It has been proposed to play a role in the modulation of neuronal responses to γ-aminobutyric acid (GABA). GABA-mediated fasthyperpolarizing inhibition depends on extrusion of chloride by KCC2 In conditions of neuronal damage, where neuronal excitability is increased, and in spinal cord in models of neuropathic pain, the expression of the KCC2 transporter is decreased |
| Immunogen: | N-terminal His-tag fusion protein corresponding to residues 932-1043 of rat K+/Cl- Cotransporter (KCC2). |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |