Anti-CSTF1 (C-terminal) antibody produced in rabbit
SIGMA/C2372 - ~1 mg/mL, affinity isolated antibody, buffered aqueous solution
Synonym: Anti-Cleavage stimulation factor, 3-prime PRE-RNA, subunit 1, 50-KD; Anti-CstF-50; Anti-CstFp50
Product Type: Chemical
Western Blotting K562 lysates were separated on SDS-PAGE and probed with Anti-CSTF1 (C-terminal) antibody produced in Rabbit (Cat. No. C2372). The antibody was developed using 1:30,000 Anti-Rabbit IgG (whole molecule)-Peroxidase antibody produced in Goat (Cat. No. A0545). Lanes 1. 2 μg/mL antibody 2. 3 μg/mL antibody
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~48 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): 5-10 μg using K562 lysates |
| western blot: 2-4 μg/mL using K562 lysates | |
| UniProt accession no. | Q05048  |
| Application: | Anti-CSTF1 (C-terminal) antibody produced in rabbit is suitable for immunoprecipitation at a working amount of 5-10μg using K562 lysates and western blotting at a concentration of 2-4μg/mL using K562 lysates. |
| Biochem/physiol Actions: | Anti-CSTF1 (C-terminal) specifically recognizes human CSTF1 (also known as Cleavage stimulation factor). |
| Biochem/physiol Actions: | Cleavage stimulation factor (50kDa) subunit is a protein encoded by the CSTF1 gene in humans. It is one of the multiple factors that plays a significant role during polyadenylation of mammalian pre-mRNAs. The mRNA polyadenylation factor CstF interacts with the BRCA1-associated protein BARD1 resulting in repression of the nuclear mRNA polyadenylation machinery. It participates in the regulation of endogenous transcripts during DNA-damage. |
| Biochem/physiol Actions: | CSTF1 is required for in vitro cleavage activity. The WD-40 repeats are crucial for the interaction with CSTF3. CSTF1 also interacts directly with the RNA polymerase II CTD, suggesting a role in linking transcription and mRNA 3′-ends processing. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Cleavage stimulation factor 1 (CSTF1) contains seven WD-40 repeats. The CSTF1 gene is mapped to human chromosome 20q13.2-q13.31. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Preparation Note: | Store at –20 °C. For continuous use, the product may be stored at 2–8 °C for up to one month. For extended storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours. |
| WGK Germany | 1 |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |