Creatinase from microorganisms
SIGMA/C2409 - lyophilized powder, ≥4 units/mg solid
Synonym: Creatine Amidinohydrolase
CAS Number: 37340-58-2
EC Number: 253-471-1
MDL Number: MFCD00130875
Product Type: Chemical
Figure 1. Stability (powder form); kept under dry conditions
Figure 2. Stability (liquid form); in 50 mM K-phosphate buffer, pH 7.5
Figure 3. pH Activity; 37 °C, 10-min. reaction in 50 mM K-phosphate buffer
Figure 4. Temperature activity; 10-min. reaction in 50 mM K-phosphate buffer, pH 7.5
Figure 5. pH Stability; 25 °C, 16-hr treatment with 50 mM buffer solution: pH 4.0–5.5, Acetate; pH 6.0–9.0, K-phosphate
Figure 4. Thermal stability; 30-min. treatment with 50 mM K-phosphate buffer, pH 7.5
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: C2409-1KU
| biological source | bacterial (Actinobacillus spp.) |
| form | lyophilized powder |
| mol wt | ~100 kDa |
| Quality Level | 200  |
| specific activity | ≥4 units/mg solid |
| storage temp. | −20°C |
| Application: | Creatinase mixed with sarcosine oxidase may be used to determine the level of creatine in different pH, temperature, enzyme ratio, and buffer concentration. It may also be used to determine the plasma creatinine level by using a centrifugal analyser. |
| Biochem/physiol Actions: | Creatinase accelerates the conversion reaction of creatine and water molecule to sarcosine and urea. It always acts in homodimer state and is induced by choline chloride. |
| General description: | Creatinase is a homodimer that catalyzes hydrolysis of creatine. It consists of two monomer subunits and two defined domains; N and C terminal domains. The C-terminal fold has both the α helices and anti-parallel β sheet within two structurally similar domains.In between these two domains, a sulfhydryl group acts as active site, and the activity is metal-independent. |
| General description: | Isoelectric point: 4.6 ± 0.1 Michaelis constant: 1.9 x 10‾2M (Creatine) Structure: 2 subunits per mole of enzyme Inhibitors: Cu++, Hg++, Ag+ Optimum pH: 8.0 Optimum temp: 40°C pH Stability: pH 5.5 − 9.0 (25°C, 16hr) Thermal stability: Below 50°C (pH 7.5, 30 min) |
| Other Notes: | One unit will hydrolyze 1.0 μmole of creatine to urea and sarcosine per min at pH 7.5 at 37 °C. |
| Packaging: | 1000 units in poly bottle |
| Physical form: | Lyophilized powder containing sugars and EDTA as stabilizers |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥4 units/mg solid |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 3.5.3.3 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |