Cytochrome c from Saccharomyces cerevisiae
SIGMA/C2436 - ≥85% based on Mol. Wt. 12,588 basis
CAS Number: 9007-43-6
EC Number: 232-700-9
MDL Number: MFCD00130890
Product Type: Chemical
The electron transport protein complex of the inner mitochondrial membrane
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: C2436-100MG
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: C2436-25MG
| application(s) | cell analysis |
| assay | ≥85% based on Mol. Wt. 12,588 basis |
| biological source | Saccharomyces cerevisiae |
| form | powder |
| mol wt | 12,588 Da |
| Quality Level | 200  |
| solubility | water: 10 mg/mL, clear, red to red-brown (dark red) |
| storage temp. | −20°C |
| technique(s) | cell based assay: suitable |
| UniProt accession no. | P00044  |
| Analysis Note: | Care has been taken to maintain the native form of the protein. It has not been artificially oxidized or reduced during purification. Contains variable amounts of reduced cytochrome c. |
| Application: | Cytochrome c from Saccharomyces cerevisiae has been used in oocyte injection to induce caspase-3 activation and p38 MAPK (mitogen-activated protein kinase). It has also been used to analyze the aerobic reactions of nitric oxide. |
| Biochem/physiol Actions: | Cytochrome c (Cytc) participates in mitochondrial electron transport and intrinsic type II apoptosis. It plays a key role in aerobic energy production. This multi-functional enzyme serves as a single electron carrier. Cytc helps in the formation of the apoptosome. |
| Biochem/physiol Actions: | The ready fluctuation of cytochrome c within the cell between ferrous and ferric states, makes it an efficient biological electron-transporter. It plays a vital role in cellular oxidations in both plants and animals. Generally regarded as a universal catalyst of respiration, it forms the essential electron-bridge between the respirable substrates and oxygen. |
| Biochem/physiol Actions: | The ready fluctuation of cytochrome c within the cell between ferrous and ferric states, makes it an efficient biological electron-transporter. It plays a vital role in cellular oxidations in both plants and animals. Generally regarded as a universal catalyst of respiration, it forms the essential electron-bridge between the respirable substrates and oxygen. Tracking cellular localization of cytochrome c in S. cerervisiae demonstrates that mitochondria are strongly involved in programmed cell death, a process very similar to apoptosis in higher organisms. |
| General description: | Cytochrome c (Cytc) comprises 104 amino acids and is a nuclear-encoded mitochondrial protein. This peripheral membrane protein is in spherical shape and has a molecular weight of 12,000 Da. |
| Other Notes: | View more information on cytochrome c and electron transport at www.sigma-aldrich.com/enz. |
| Packaging: | 25, 100 mg in poly bottle |
| Preparation Note: | Prepared without using TCA. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥85% based on Mol. Wt. 12,588 basis |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |