Cytochrome c from equine heart
SIGMA/C2506 - ≥95% (SDS-PAGE)
Synonym: Cytochrome c from horse heart
CAS Number: 9007-43-6
EC Number: 232-700-9
MDL Number: MFCD00130890
Product Type: Chemical
Cytochrome c is a heme protein containing a single polypeptide chain and a single heme group, which is covalently attached to Cys14 and Cys17.
The electron transport protein complex of the inner mitochondrial membrane
Cytochrome c from equine heart (Cat. No. C2506) was dissolved in 1× denaturing Sample Buffer, heated at 85 °C for 2 minutes, run on a 4–20% Tris-glycine gel in 1× Tris-Glycine Buffer.
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: C2506-5G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: C2506-2G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: C2506-100MG
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: C2506-250MG
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: C2506-50MG
| application(s) | cell analysis |
| assay | ≥95% (SDS-PAGE) |
| biological source | horse heart |
| form | powder |
| mol wt | 12,384 |
| Quality Level | 300  |
| solubility | H2O: soluble 10 mg/mL |
| storage temp. | −20°C |
| suitability | suitable for molecular biology |
| technique(s) | cell based assay: suitable |
| UniProt accession no. | P00004  |
| Application: | Cytochrome c has been used:• in the reduction of ferricytochrome c by xanthine oxidase-generated superoxide radicals • as an analyte by the single droplet deposition method using MALDI mass spectra • as a terminal electron acceptor in the assay of standard cytochrome c reductase activity • in cytochrome c oxidase histochemistry • in the measurement of cytochrome C oxidase activity • as a component of phosphate buffer for the detection and evaluation of complex IV activity using blue-native gel electrophoresis (BN-PAGE) • for detection of extracellular superoxide anion (ECSA) in isolated kidney phagocytes |
| Application: | The specific sites and extent of oxidation in horse cytochrome c treated with H2O2 and UV were characterized. It was suggested that the state of these sites could be used as a biomarker for the oxidative environment in a cell. |
| Biochem/physiol Actions: | Cytochrome c is primarily known as an electron-carrying mitochondrial protein. The transition of cytochrome c between the ferrous and ferric states within the cell makes it an efficient biological electron-transporter and it plays a vital role in cellular oxidations in both plants and animals. It is generally regarded as a universal catalyst of respiration, forming an essential electron-bridge between the respirable substrates and oxygen |
| Biochem/physiol Actions: | Cytochrome c, released from the outer mitochondrial compartment into the cytosol aids in activation of caspase-9 and initiation of the apoptotic protease cascade. This process is facilitated through its interaction with apoptotic protease-activating factor-1 (APAF-1) and deoxyadenosine triphosphate (dATP).(3) Various studies suggest that measurement of cytochrome C level in serum of tumor patients can be considered as a potential marker for cell death in vivo. |
| Other Notes: | 2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research |
| Other Notes: | View more information on cytochrome c and electron transport at www.sigma-aldrich.com/enz. |
| Packaging: | 1, 2, 5 g in glass bottle |
| Packaging: | 50, 100, 250, 500 mg in glass bottle |
| Preparation Note: | Prepared using TCA. |
| Preparation Note: | This product in solution should be dark red to reddish brown when dissolved in water at 10 mg/mL. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥95% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |