Carbonic Anhydrase Isozyme II from bovine erythrocytes
SIGMA/C2522 - lyophilized powder, ≥3,000 W-A units/mg protein
Synonym: Carbonate Dehydratase; Carbonate Hydrolyase; Carbonic Anhydrase II
CAS Number: 9001-03-0
EC Number: 232-576-6
MDL Number: MFCD00081467
Product Type: Chemical
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| biological source | bovine erythrocytes |
| form | lyophilized powder |
| mol wt | 30 kDa |
| pI | ~5.9 |
| Quality Level | 300  |
| specific activity | ≥3,000 W-A units/mg protein |
| storage temp. | −20°C |
| Application: | Carbonic anhydrase from sigma has been used for the analysis of thermodynamic stability of the enzyme by measuring the binding affinity and stoichiometry. The enzyme has also been used to generate CD4+ T cell lines specific for carbonic anhydrase during the study of autoimmune pancreatitis in rat. |
| Biochem/physiol Actions: | Carbonic anhydrase is a zinc metalloenzyme that has a molecular weight of approximately 30,000 Da. The enzyme catalyzes the hydration of carbon dioxide to carbonic acid. It is involved in many physiological and pathological processes such as pH and CO2 homeostasis, transport of bicarbonate and CO2, biosynthetic reactions, bone resorption, calcification, and tumorigenicity. Therefore, inhibitors that block this enzyme can have therapeutic implications in glaucoma, epilepsy and Parkinson′s disease. |
| Other Notes: | One Wilbur-Anderson (W-A) unit will cause the pH of a 0.02 M Trizma buffer to drop from 8.3 to 6.3 per min at 0 °C. (One W-A unit is essentially equivalent to one Roughton-Booth unit.) |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥3,000 W-A units/mg protein |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 4.2.1.1 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |