Monoclonal Anti-N-Cadherin antibody produced in mouse
SIGMA/C2542 - clone GC-4, ascites fluid
Synonym: Anti-A-CAM; Anti-A-Cell Adhesion Molecule
MDL Number: MFCD00164511
Product Type: Chemical
Immunohistochemistry Frozen sections, acetone fixed Chicken cardiac muscle were stained with 1:200 Monoclonal Anti-N-Cadherin antibody produced in Mouse, Clone: GC-4 (Cat. No. C2542), followed by 1:200 Anti-Mouse IgG (Fab specific)-FITC antibody produced in Goat (Cat. No. F5262).
Western Blotting Whole extract Chicken cardiac muscle were separated on SDS-PAGE and probed with Monoclonal Anti-N-Cadherin antibody produced in Mouse, Clone: GC-4 (Cat. No. C2542). The antibody was developed using 1:10,000 Anti-Mouse IgG (Fab specific)-Peroxidase antibody produced in Goat (Cat. No. A9917). Lanes 1. 1:100 antibody 2. Negative Control
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| application(s) | research pathology |
| biological source | mouse |
| clone | GC-4, monoclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | chicken, mouse, human, rabbit, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | electron microscopy: suitable |
| immunohistochemistry (frozen sections): 1:100 using chicken cardiac muscle | |
| microarray: suitable | |
| western blot: 1:100 using chicken or rat cardiac muscle | |
| UniProt accession no. | P19022  |
| Application: | Monoclonal anti-N-cadherin antibody can be used in immunofluorescent microscopy, electron microscopy and immunoblotting to study the receptor localization of intercellular adherens. It may also be used to study the mechanism of adhesion and interaction in tissues. |
| Application: | Monoclonal anti-N-cadherin antibody can be used in immunohistochemistry (1: 100) and as primary antibody in western blotting (1:1000) . |
| Biochem/physiol Actions: | Monoclonal anti-N-cadherin antibody reacts specifically with A-CAM molecule in human, mouse, rabbit, chicken and rat. |
| Biochem/physiol Actions: | N-cadherin regulates the Src kinase pathway and also promotes cell motility and invasion. It inhibits adherens junction formation and disrupts existing junctions of cultured lens cells. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | N-cadherin is a calcium dependent cell adhesion molecule and is present mainly in cancer cells. Monoclonal anti-N-cadherin antibody reacts with the N-terminal portion of the extracellular domain of adipocyte adhesion molecule (ACAM). |
| Immunogen: | Affinity purified chicken heart A-CAM. |
| Physical form: | Monoclonal Anti-N-Cadherin antibody produced in mouse is supplied as ascites fluid containing 15mM sodium azide. |
| Preparation Note: | For continuous use, store at 2-8 °C for up to one month. For extended storage, solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify by centrifugation before use. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |