Anti-Human IgG (Fc specific)-Cy3 antibody produced in goat
SIGMA/C2571 - affinity isolated antibody, buffered aqueous solution
MDL Number: MFCD00212909
Product Type: Chemical
Double immunofluorescent labeling PTK-1 cells in anaphase and interphase stained with Monoclonal and Anti-β-Tubulin (clone no. 2-28-33, Cat. No. T5293 and Goat Anti-Mouse IgG-FITC, F(ab′)2 fragment (Cat. No. F8521) and anti-centromere from human sera with Goat Anti-Human IgG-Cy3 (Cat. No. C2571). Chromosomes are counterstained with Hoechst (Cat. No. B2261). Images were acquired on a videomicroscope with a cooled CCD camera. From J. De Mey and P. Guillaud, Institute Jacques Monod, Paris.
| antibody form | affinity isolated antibody |
| antibody product type | secondary antibodies |
| biological source | goat |
| clone | polyclonal |
| conjugate | CY3 conjugate |
| form | buffered aqueous solution |
| Quality Level | 200  |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50 |
| Application: | Anti-Human IgG (Fc specific)-Cy3 antibody produced in goat was used to screen sera on glycopeptide microarrays at a dilution of 1:1000. It was used at a working dilution of 1:100 for immunohistochemistry analysis of mouse footpad sections incubated with human polyclonal anti-Leishmania serum. |
| Biochem/physiol Actions: | IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections. The coupling of Cy3 to Anti-Human IgG (Fc specific) antibody allows for the visualization of proteins by fluorescent microscopy. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Legal Information: | Cy is distributed under license from Amersham Biosciences Limited. |
| Packaging: | 1 mL in glass bottle |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |