Anti-Calbindin-D-28K (EG-20) antibody produced in rabbit
SIGMA/C2724 - affinity isolated antibody, buffered aqueous solution
Synonym: Anti-CALB; Anti-D-28K
MDL Number: MFCD00164553
Product Type: Chemical
Enhanced Validation-By Independent Antibodies Immunohistochemistry. Formalin-fixed, paraffin-embedded Rat Cerebellum sections stained with 1:3,000 Monoclonal Anti-Calbindin-D-28K antibody produced in Mouse, Clone: CB-955 (Cat. No.C9848) (A). The antibody was developed using 20 μg/mL Goat Anti-Rabbit IgG (whole molecule)-Biotin (Cat. No. B8895) followed by 20 μg/mL ExtrAvidin®-Peroxidase (Cat. No. E2886), and 1:2,000 Anti-Calbindin-D-28K (EG-20) antibody produced in Rabbit (Cat. No. C2724) (B). The antibody was developed using 20 μg/mL Goat Anti-Rabbit IgG (whole molecule)-Biotin (Cat. No. B8895) followed by 20 μg/mL ExtrAvidin®-Peroxidase (Cat. No. E2886). Results Two Anti-Calbindin-D-28K antibodies, C9848 (A) and C2724 (B), target different regions of Calbindin-D-28K show similar staining profiles between the two antibodies, demonstrating Independent Antibody Verification.
Immunohistochemistry Staining of formalin-fixed, paraffin-embedded Rat cerebellum sections with 1:2,000 Rabbit Anti-Calbindin-D-28K (EG-20) (Cat. No. C2724) using biotin/ExtrAvidin®-Peroxidase.
Western Blotting Tissue lysates were separated on SDS-PAGE and probed with 1:2,000 Rabbit Anti-Calbindin-D-28K (EG-20) (Cat. No. C2724). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. Rat Brain 2. Rat Kidney
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| enhanced validation | independent Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous solution |
| mol wt | antigen 28 kDa |
| Quality Level | 200 |
| shipped in | dry ice |
| species reactivity | rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: 1:2,000 using sections of rat cerebellum |
| western blot: 1:2,000 using a whole extract of rat brain | |
| UniProt accession no. | P07171  |
| Analysis Note: | Enzymatic predigestion of formalin-fixed, paraffin-embedded sections by proteolytic enzymes (e.g., 0.1% trypsin or protease, 10 min at RT or 37 °C) improves immunohistochemical staining. |
| Application: | Anti-Calbindin-D-28K (EG-20) antibody is suitable for use in immunohistochemistry (1:2,000 using sections of rat cerebellum), immunoblot and western blot (1:2,000 using a whole extract of rat brain). Enzymatic predigestion of formalin-fixed, paraffin-embedded sections by proteolytic enzymes (e.g., 0.1% trypsin or protease, 10 min., at RT or 37 ° C) improves immunohistochemical staining with the antibody. Anti-Calbindin-D-28K (EG-20) antibody has been used in immunohistochemical (IHC) peroxidase staining and cupric silver staining. It has also been used in immunofluorescence. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Calbindin-D-28K (CALB1) is a calcium binding protein in the brain. This 27kDa protein is located on human chromosome 8q21. |
| General description: | Calbindin-D-28k is an intracellular protein characterized by EF-hand type structural motifs that bind to Ca2+. This protein modulates synaptic plasticity and signal transduction . Anti-Calbindin-D-28K (EG-20) antibody recognizes rat calbindin-D-28K (28 kDa). Due to interspecies similarity, reactivity with human, mouse, bovine, chicken, porcine, and frog is expected, but has not been confirmed. Staining of calbindin-D-28K is specifically inhibited with the calbindin-D-28K immunizing peptide by immunoblotting. |
| Immunogen: | synthetic peptide corresponding to the C-terminal region of rat calbindin-D-28K (amino acids 225-244 with N-terminally added Lys-Gly) conjugated to KLH. |
| Immunogen: | The immunogen sequence is specific for calbindin-D-28K and is not found in other members of the EF-hand family such as calbindin-D-9K, calretinin, myosin light chain, parvalbumin, S-100a, S-100b, S100A2 (S100L) and S100A6 (calcyclin). This sequence is highly conserved across species, including human, mouse, bovine, chicken, porcine, and frog calbindin-D-28K sequences. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |