Monoclonal Anti-I-CAM-1 (CD54) antibody produced in mouse
SIGMA/C2969 - clone 8.4A6, purified immunoglobulin
Synonym: Monoclonal Anti-CD54; Anti-I-CAM-1
MDL Number: MFCD00240668
Product Type: Chemical
Immunohistochemistry Frozen sections, acetone fixed Human tonsil were stained with 20 μg/mL Monoclonal Anti-I-CAM-1 (CD54) antibody produced in Mouse, Clone: 8.4A6 (Cat. No. C2969), followed by 1:20 Anti-Mouse IgG (Fab specific)-FITC antibody produced in Goat (Cat. No. F5262).
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 8.4A6, monoclonal |
| conjugate | unconjugated |
| isotype | IgG1 |
| mol wt | antigen 85-110 kDa |
| shipped in | wet ice |
| species reactivity | human, monkey |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | flow cytometry: suitable |
| immunocytochemistry: suitable | |
| immunohistochemistry (frozen sections): suitable | |
| indirect ELISA: suitable | |
| UniProt accession no. | P05362   |
| Biochem/physiol Actions: | The antibody recognizes the human CD54 (I-CAM-1) surface antigen by flow cytometry, ELISA, immunocytochemistry, and immunohistochemistry. It inhibits human leukocyte adhesion to activated endothelial cells and stimulates homotypic aggregation of SKW3 cells. The epitope recognized by the antibody is localized in domain D2 of the CD54 molecule. 5th Workshop: code no. S 100 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | CD54 (I-CAM-1) is a glycoprotein involved in mediation of intercellular adhesion. CD54 is expressed by activated endothelial cells and is also detected on cells of many other lineages and in plasma. |
| Immunogen: | TNF-α activated human endothelial cells. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4. |
| Preparation Note: | Product filtered through a 0.2 μm filter |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |