Monoclonal Anti-Cathepsin L antibody produced in mouse
SIGMA/C2970 - clone 33/2, ascites fluid
Synonym: Anti-CATL; Anti-CTSL; Anti-MEP
MDL Number: MFCD01321805
Product Type: Chemical
Western Blotting JOURNAL CITATION: Astaxanthin intake attenuates muscle atrophy caused by immobilization in rats. By: Shibaguchi, T., Yamaguchi, Y., et al. in Physiol Rep, 2016. PubMed ID: 27482075 Image collected and cropped by CiteAb from the following publication, (http://doi.wiley.com/10.14814/phy2.12885), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Monoclonal Anti-Cathepsin L, Clone 33/2 (C 2970)
Immunoblot
Whole cell extract of Mv 1 Lu cultured mink lung was separated on SDS-PAGE and blotted with Monoclonal Anti-Cathepsin L (Product No. C 2970). The antibody was developed with Goat Anti-Mouse IgG (Fab), AlkPhos Conjugate (Product No. A 1293) and NBT/BCIP substrate.
Lane A: Antibody dilution 1:400
Lane B: Negative control (only secondary antibody)
Immunoblot
Whole cell extract of Mv 1 Lu cultured mink lung was separated on SDS-PAGE and blotted with Monoclonal Anti-Cathepsin L (Product No. C 2970). The antibody was developed with Goat Anti-Mouse IgG (Fab), AlkPhos Conjugate (Product No. A 1293) and NBT/BCIP substrate.
Lane A: Antibody dilution 1:400
Lane B: Negative control (only secondary antibody)
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 33/2, monoclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| isotype | IgG1 |
| mol wt | antigen (human cathepsin L) 25 kDa |
| antigen (human procathepsin L) 42 kDa | |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | mouse, mink, human, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (frozen sections): suitable |
| indirect ELISA: suitable | |
| microarray: suitable | |
| western blot: 1:200 using whole cell extract of a cultured mink lung cell line | |
| UniProt accession no. | P07711  |
| Biochem/physiol Actions: | Cathepsin L expression is correlated with the metastatic potential of transformed cells. Cathepsin L is capable of degrading protein constituents of the extracellular matrix, this enzyme is thought to play a crucial role in tumor progression, metastasis and other disorders where the destruction of the matrix is the major cause of disease. |
| Biochem/physiol Actions: | Reacts specifically with native and denatured forms of human cathepsin L and procathepsin L. Recognizes an epitope within amino acid residues GYGFEST (265-271 in procathepsin L and 169-175 in the mature cathepsin L molecule). The antibody may be used for the suppression of the malignant growth of myeloma cells. Cross-reactivity is observed with rat cathepsin L (strong), mouse procathepsin L (weak) and mink cathepsin and procathepsin L. Does not cross-react with human cathepsin types B, D, H and S, procathepsins B and D, rat cathepsin B, and mouse procathepsins B and D. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Cathepsin L is a cysteine protease and is secreted by numerous transformed cells in its inactive pre-form. It is ubiquitously expressed and is localized in nucleus. Cathepsin L have substrate interacting region located in L and R domain loops. |
| Immunogen: | procathepsin L isolated from the human lung cancer cell line EPLC 32M1. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |