CelLytic™ M
SIGMA/C2978 - Cell Lysis Reagent, Suitable for Mammalian cell lysis and protein solubilization.
Synonym: Whole-cell protein extraction solution
Product Type: Chemical
Figure 1. Comparison of Extraction Efficiency.
2 x 107 COS cells were washed and divided into equal aliquots, then lysed by one of the methods indicated. Protein amounts were determined by a BCA assay.
2 x 107 COS cells were washed and divided into equal aliquots, then lysed by one of the methods indicated. Protein amounts were determined by a BCA assay.
| form | solution |
| Quality Level | 200  |
| storage temp. | room temp |
| Analysis Note: | Use 125 μl CelLytic M for 106-107 of suspended cells. For adherent cells, use 500-1,000 μL for a 100 mm plate; 200-400 μL for a 35 mm plate. |
| Features and Benefits: | • Efficient: Up to 50% more efficient than freeze thaw, sonication and other products • Non-denaturing: Does not interfere in downstream applications such immunoprecipitation, kinase and phosphatase assays, reporter gene assays and gel shift assays • Convenient: Ready-to-use reagent requires no scraping from culture plates • Fast: Rapid cell lysis at room temperature. |
| General description: | CelLytic M is a proprietary detergent solution designed for efficient whole-cell protein extraction from cultured mammalian cells. It enables efficient and rapid cell lysis and solubilization of proteins for both suspension and adherent cells. Lysates can be used in many downstream applications without removing the CelLytic M such as reporter gene assays, Western blots/immunoprecipitation |
| Legal Information: | CelLytic is a trademark of Sigma-Aldrich Co. LLC |
| Preparation Note: | CelLytic M reagent efficiency for protein extraction has been tested on, but not limited to, HeLa, CHO, COS, HL-60, Jurkat, A431, PC-12, and Bovine Aorta Endothelial Cells (BAEC). |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | room temp |
| UNSPSC | 41116134 |