Anti-CRMP2 antibody produced in rabbit
SIGMA/C2993 - ~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution
Synonym: Anti-Collapsin Response Mediator Protein 2; Anti-
MDL Number: MFCD03456049
Product Type: Chemical
Enhanced Validation-By Recombinant Expression Antibody recognition: Anti-CRMP2 antibody produced in Rabbit (Cat. No. C2993) is confirmed by western blot in the cell sample that has been altered to overexpress the target protein-HEK-293T over expressing CRMP2 (Lane 1) while no staining is detected in the wild type sample-HEK-293T (Lane 2). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and a chemiluminescent substrate. Antibody dilution: 1:10,000.
Immunoblotting Tissue extracts and whole cell lysates were separated on SDS-PAGE and blotted with Anti-CRMP2 (Cat. No. C2993). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and a chemiluminescent substrate. Antibody dilution 1:10,000 Lanes 1,6: Mouse brain (S1 fraction) 2: Rat brain (S1 fraction) 3: HeLa cells 4: HEK-293T cells transfected with human CRMP2 5: Untransfected HEK-293T cells 6: Inhibition with CRMP2 immunizing peptide (human 476-493) (10 μg/mL)
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous solution |
| mol wt | antigen ~62 kDa |
| Quality Level | 200 |
| shipped in | dry ice |
| species reactivity | human, mouse, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | western blot: 0.1-0.2 μg/mL using HeLa whole cell lysate and mouse brain extract (S1 fraction) |
| UniProt accession no. | Q16555  |
| Application: | Anti-CRMP2 antibody produced in rabbit has been used in: • western blotting • immunohistofluorescence • epifluorescence imaging • coimmunoprecipitation |
| Biochem/physiol Actions: | CRMPs are a part of the collapsin/semaphorin signal transduction pathway implicated in semaphorin-induced growth cone collapse during neural development. CRMP2 is upregulated during development, and appears to be crucial for axon outgrowth. Glycogen synthase kinase 3 beta (GSK-3b) phosphorylates and inactivates CRMP-2 downstream of the phosphoinositide-3-kinase (PI3K/Akt) pathway, thus regulating neuronal polarity. CRMP2 interacts with tubulin dimers, kinesin-1 and WASP-family verprolin homologous protein 1 (WAVE1) complex to regulate axon outgrowth. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Collapsin response mediator proteins CRMPs) consist of a family of cytosolic phosphoproteins expressed in the nervous system and involved in neuronal differentiation and axonal guidance. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |