Catalase from bovine liver
SIGMA/C30 - aqueous suspension, 10,000-40,000 units/mg protein
Synonym: Bovine hepatocatalase; H2O2:H2O2 oxidoreductase
CAS Number: 9001-05-2
MDL Number: MFCD00081483
Product Type: Chemical
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| biological source | bovine liver |
| concentration | 20-50 mg/mL |
| form | aqueous suspension |
| InChI | 1S/C9H10O3/c1-2-12-9(11)7 |
| InChI key | NUVBSKCKDOMJSU-UHFFFAOYSA |
| isoelectric point | 5.4 |
| mol wt | tetramer ~250 kDa |
| Quality Level | 200  |
| shipped in | wet ice |
| SMILES string | O(CC)C(=O)c1ccc(cc1)O |
| specific activity | 10,000-40,000 units/mg protein |
| storage temp. | 2-8°C |
| technique(s) | GC/MS: suitable |
| UniProt accession no. | P00432  |
| Application: | Catalase acts as a natural antioxidant to study the roles of reactive oxygen species in gene expression and apoptosis. It has also been used to protect against oxidative damage to proteins, lipids, and nucleic acids. Industrially, catalzes have been used to remove hydrogen peroxide added to milk and cheese, in textile bleaching, and to examine its positive effects on the viability of DNA-repair mutants of E. coli. Catalase from bovine liver may be used:
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| Application: | Catalase from bovine liver has been used for measuring the hydrogen peroxide content in cancer tissue homogenates. It has also been used to test the effect of organophosphate insecticide chlorpyrifos-ethyl (CE) [0,0-diethyl 0 (3,5,6-trichloro-2-pyridy |
| Application: | Catalase from bovine liver is used for the following applications: • Dielectrophoretic field-flow fractionation (DEP-FFF): It is a chromatographic method in which cell elution times reflect the positions of cells in a hydrodynamic flow profile under the control of sedimentation, DEP and hydrodynamic lift forces, FSED, FDEP and FHDL, respectively. The DEP-FFF buffer consists of catalase along with other ingrediants. • GC-MS analyses. • Deionization. • GC-FID analysis.5 • Functional analysis of blood vessel. |
| Biochem/physiol Actions: | Catalase, an antioxidant enzyme found in all aerobic organisms, catalyzes the degradation of hydrogen peroxide, a byproduct of metabolic processes, into less harmful water and oxygen. It can also react with alkylhydrogen peroxides, such as methylperoxide and ethylperoxide and the second H2O2 molecule can be replaced by methanol, ethanol, propanol, formate and nitrate as a hydrogen donor. Catalase enzyme uses either iron (Fe) or manganese (Mn) as cofactor, and are classified as Fe-CAT or Mn-CAT. |
| Biochem/physiol Actions: | This product doesn′t need any activators, but it is inhibited by 3-amino-1-H-1,2,4 triazole, cyanide, azide, hydroxylamine, cyanogens bromide, 2-mercaptoethanol, dithiothreitol, dianisidnie and nitrate. |
| Disclaimer: | Solutions of catalse should not be frozen. Frozen solution will result in a 50-70% loss of activity. |
| General description: | Catalase from bovine liver contains 506 residues. It is a tetramer and each monomer corresponds to a molecular weight of 61 kDa. The active site in each momomer comprises nicotinamide adenine dinucleotide phosphate (NADPH) and iron binding region. Research area: Cell Signaling |
| General description: | Research Area: Cell Signaling Catalase from bovine liver is a tetramer consisting of 4 equal subunits each with a 60 kDa molecular weight. Each of these subunits contains iron bound to a protoheme IX group. The enzyme will also strongly bind to NADP, where NADP and the heme group are within 13.7 angstroms. |
| Other Notes: | One unit will decompose 1.0 μmole of H2O2 per min at pH 7.0 at 25 °C, while the H2O2 concentration falls from 10.3 to 9.2 mM, measured by the rate of decrease of A240. |
| Preparation Note: | This product is a crystalline suspension in water containing 0.1% thymol with activity of 10,000-40,000 units/mg. |
| Preparation Note: | Tightly closed. Dry. Keep locked up or in an area accessible only to qualified or authorized |
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H334 |
| Precautionary statements | P261 - P284 - P501 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: 10,000-40,000 units/mg protein |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 1.11.1.6 ( BRENDA | IUBMB ) |
| UNSPSC | 12352204 |