Colipase from porcine pancreas
SIGMA/C3028 - essentially salt-free, lyophilized powder
Synonym: Colipase from hog pancreas
CAS Number: 55126-92-6
EC Number: 259-490-1
MDL Number: MFCD00130823
Product Type: Chemical
SDS-PAGE Purity of Colipase from Porcine pancreas (Cat. No. C3028) was assessed on SDS-PAGE.
| application(s) | food and beverages |
| assay | ≥95% (Lowry) |
| biological source | Porcine pancreas |
| color | white to faint yellow |
| concentration | ≥500 μ protein/vial |
| form | essentially salt-free, lyophilized powder |
| Quality Level | 200  |
| solubility | H2O: soluble 1.9-2.1 mg/mL, clear, colorless to faintly yellow |
| storage temp. | −20°C |
| suitability | suitable for molecular biology |
| UniProt accession no. | P02703  |
| Application: | Colipase from porcine pancreas has been used: • in the enzyme solution for lipase assay • in an enzyme mixture to simulate gastrointestinal digestion • as one of the apparent critical factors to study its effect on lycopene in vitro accessibility |
| Biochem/physiol Actions: | Colipase is involved in lipid metabolism and apoptosis signaling. It is crucial for fat digestion. It is a pancreatic protein that prevents the denaturation of lipase and enables its attachment to the lipid-water interphase of the droplet. Colipase makes lipase accessible to the inner core of triacylglycerol. Defective colipase secretion induced by an insufficient exocrine pancreatic function minimizes the luminal hydrolysis of dietary fat. Lack of congenital lipase or colipase causes pancreatic fat malabsorption. |
| Biochem/physiol Actions: | Colipase, a small protein cofactor, is essential for efficient dietary lipid hydrolysis by pancreatic lipase. It attaches to the non-catalytic C-terminal domain of the lipase, stabilizing an active conformation and significantly enhancing the overall hydrophobic binding site. This pancreatic exocrine protein aids in the adsorption of pancreatic triglyceride lipase (PTL) to the substrate lipid-water interface. |
| Features and Benefits: | • Overcomes the inhibition of lipase by bile salts. • Activity can be demonstrated turbidimetrically in an emulsion of triolein and sodium deoxycholate together with porcine pancreatic lipase. |
| General description: | Research Area: Cell Signaling Colipase is a member of a group of small cysteine-rich proteins characterized by a limited secondary structure. Its structure is relatively flat, measuring 25×30×35 Å, resembling an arrangement of protruding fingers interconnected at one end by a complex network of five disulfide bridges. The colipase structure comprises two small domains with closely related topology. |
| General description: | The gene colipase (CLPS) encodes a lipolytic enzyme that is majorly expressed in the exocrine pancreas. This protein is also found in the stomach and intestine. Its structure includes 17 amino acid signal peptides and a five amino acid pro-piece at the N-terminus. |
| Packaging: | 0.5 mg in serum bottle |
| Preparation Note: | Dissolves in water to form a clear, colorless to faint yellow colored solution at 1.9-2.1 mg/mL concentration. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥95% (Lowry) |
| Storage Temp. | −20°C |
| UNSPSC | 12352204 |