Cytochrome c from bovine heart
SIGMA/C3131 - ≥95% based on Mol. Wt. 12,327 basis, powder, suitable for mammalian cell culture
Synonym: CYC; CytC
CAS Number: 9007-43-6
EC Number: 232-700-9
MDL Number: MFCD00130890
Product Type: Chemical
The electron transport protein complex of the inner mitochondrial membrane
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: C3131-100MG
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: C3131-250MG
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: C3131-50MG
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: C3131-1G
| assay | ≥95% based on Mol. Wt. 12,327 basis |
| biological source | bovine heart |
| form | powder |
| impurities | 0.40-0.50% Iron (anhydrous) |
| mol wt | 12327 Da |
| Quality Level | 200  |
| solubility | water: 10 mg/mL, dark red-brown |
| storage condition | (Tightly closed Dry) |
| storage temp. | −20°C |
| technique(s) | cell culture | mammalian: suitable |
| UniProt accession no. | P62894  |
| Application: | Cytochrome C from bovine heart has been used: • as a component of the assay solution for testing the activity of respiratory chain complexes III and IV in the mitochondria of human osteosarcoma and human hepatocarcinoma cell lines • to determine the cytochrome c oxidase activity in mitochondria of human aortic endothelial cells • as a component of the cyt C oxidase reaction solution during in situ enzyme staining of cyt C oxidase in mice kidney tissues |
| Biochem/physiol Actions: | Cytochrome C (Cyt C) is involved in the activation of caspase during the caspase-dependent apoptosis intrinsic pathway that triggers programmed cell death through apoptosis. It shows lipid-binding activity through its interaction with cardiolipin that accounts for the peroxidase activity of Cyt C. Cyt C binds to heme via the help of cytochrome c heme lyase that enables its release into the mitochondrial intermembrane space. |
| Biochem/physiol Actions: | The ready fluctuation of cytochrome c within the cell between ferrous and ferric states, makes it an efficient biological electron-transporter. It plays a vital role in cellular oxidations in both plants and animals. Generally regarded as a universal catalyst of respiration, it forms the essential electron-bridge between the respirable substrates and oxygen. |
| Biochem/physiol Actions: | The ready interconversion of cytochrome c between ferrous and ferric states makes it an efficient biological electron carrier. It plays a vital role in cellular oxidations in both plants and animals. Generally regarded as a universal link in the respiratory chain, it forms the essential electron-bridge between the respirable substrates and oxygen. |
| General description: | Research area: Apoptosis Cytochrome c (Cyt C) is an essential mitochondrial protein located in the inner membrane of the mitochondria. It is encoded in the nucleus as apo-cytochrome C. Cyt C occurs as membrane-bound or as soluble metalloproteins in energy-transducing membranes and is found in bacteria, archaea, and plastids. It belongs to the group of class IIa cytochromes and contains pentacoordinate heme centres. |
| Other Notes: | View more information on cytochrome c and electron transport at www.sigma-aldrich.com/enz. |
| Packaging: | 1 g in poly bottle |
| Packaging: | 10, 50, 100, 250 mg in poly bottle |
| Preparation Note: | Prepared with acetic acid without using TCA. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥95% based on Mol. Wt. 12,327 basis |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |