Cathepsin D from bovine spleen
SIGMA/C3138 - lyophilized powder, ≥2.0 units/mg protein
Synonym: CTSD
CAS Number: 9025-26-7
MDL Number: MFCD00130749
Product Type: Chemical
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| application(s) | life science and biopharma |
| assay | 10—70% protein (biuret) |
| biological source | bovine spleen |
| color | dark brown |
| form | lyophilized powder |
| manufacturer/tradename | Sigma-Aldrich |
| mol wt | ~45 kDa |
| Quality Level | 200  |
| specific activity | ≥2.0 units/mg protein |
| storage temp. | −20°C |
| suitability | suitable for molecular biology |
| technique(s) | activity assay: suitable |
| UniProt accession no. | P80209  |
| Application: | Cathepsin D from bovine spleen has been used: • in in vitro dose-dependent fluorometric activity assays. • in in vitro myelin oligodendrocyte glycoprotein (MOG) digestion to study the uptake of malondialdehyde (MDA)-modified MOG and its implications in central nervous system autoimmunity. • for enzymatic digestion of the proteoglycan moiety of the articular cartilage in order to determine its dynamic elastic modulus at two different levels of tissue organization. • in cathepsin D activity assay. |
| Biochem/physiol Actions: | Cathepsin D is an endosomal-lysosomal aspartic protease implicated in breast cancer metastasis and Alzheimer’s disease. Lysosomal release of cathepsin D has been found to precede cytochrome c release and loss of membrane potential in apoptotic human foreskin fibroblasts. Cathepsin D levels in PC12 cells increase 12 to 24 hours after apoptosis is induced. |
| Biochem/physiol Actions: | Endosomal-lysosomal aspartic protease implicated in breast cancer metastasis and Alzheimer’s disease. Lysosomal release of cathepsin D has been found to precede cytochrome c release and loss of membrane potential in apoptotic human foreskin fibroblasts. |
| General description: | Research area: Cell Signaling Cathepsin D is a soluble endosomal-lysosomal aspartic protease and is synthesized in the rough endoplasmic reticulum as preprocathepsin D. It is encoded by the CTSD gene located in the 11p15.5 region. |
| Other Notes: | One unit will produce an increase in A280 of 1.0 per min per mL at pH 3.0 at 37 °C measured as TCA-soluble products using hemoglobin as substrate (1 cm light path). |
| Packaging: | 5, 10, 20, 40 units in serum bottle |
| Physical form: | Lyophilized powder containing citrate buffer salts |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | 10—70% protein (biuret) |
| activity | specific activity: ≥2.0 units/mg protein |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 3.4.23.5 ( BRENDA | IUBMB ) |
| UNSPSC | 12352204 |