α-Chymotrypsin from bovine pancreas
SIGMA/C3142 - (TLCK treated to inactivate residual tryspin activity), Type VII, essentially salt-free, lyophilized powder, ≥40 units/mg protein
Synonym: TLCK-Chymotrypsin
CAS Number: 9004-07-3
EC Number: 232-671-2
MDL Number: MFCD00130481
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: C3142-100MG
| form | essentially salt-free, lyophilized powder |
| mol wt | 25 kDa |
| Quality Level | 200  |
| solubility | 1 mM HCl: soluble 10 mg/mL, clear |
| specific activity | ≥40 units/mg protein |
| storage temp. | −20°C |
| type | Type VII |
| UniProt accession no. | P00767  |
| Analysis Note: | Protein determined by A |
| Application: | α-Chymotrypsin from Sigma has been used to determine the crystal structures of two homologous inhibitors (pars intercerebralis major peptide-C and pars intercerebralis major peptide-D2v) from the insect Locusta migratoria by forming a complex with the enzyme. |
| Biochem/physiol Actions: | α-Chymotrypsin is a serine peptidase and has 241 amino acid residues contained in three polypeptide chains (A chain-13 residues, B chain-131 residues, and C chain-97 residues) linked by disulfide bridges. Molecular weight of this enzyme is found to be 25 kDa. Its pI is 8.75. It selectively hydrolyzes peptide bonds on the C-terminal side of tyrosine, phenylalanine, tryptophan, and leucine. Ca2+ activates and stabilizes the enzyme. The enzyme is inhibited by diisopropyl fluorophosphate (DFP), phenylmethanesulfonyl fluoride (PMSF), N-p-tosyl-L-phenylalanine chloromethyl ketone (TPCK), chymostatin, aprotinin, α1-antitrypsin, α2-macroglobulin, 10 mM Cu2+ and Hg2+. |
| Biochem/physiol Actions: | A serine protease that hydrolyzes peptide bonds with aromatic or large hydrophobic side chains (Tyr, Trp, Phe, Met, Leu) on the carboxyl end of the bond. |
| Other Notes: | One unit will hydrolyze 1.0 μmole of BTEE per min at pH 7.8 at 25 °C. |
| Other Notes: | View more information on chymotrypsin at www.sigma-aldrich.com/enz |
| Packaging: | 10, 25, 100 mg in glass bottle |
| Preparation Note: | TLCK treatment inactivates trypsin which may be present in chymotrypsin, without affecting the chymotrypsin activity. |
| Symbol |    GHS07,GHS08,GHS09 |
| Signal word | Danger |
| Hazard statements | H302 - H315 - H319 - H334 - H335 - H400 |
| Precautionary statements | P261 - P273 - P301 + P312 - P302 + P352 - P304 + P340 + P312 - P305 + P351 + P338 |
| Hazard Codes | Xn |
| Risk Statements | 36/37/38-42 |
| Safety Statements | 22-24-26-36/37 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| activity | specific activity: ≥40 units/mg protein |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 3.4.21.1 ( BRENDA | IUBMB ) |
| UNSPSC | 12352204 |