Creatininase from microorganisms
SIGMA/C3172 - lyophilized powder, 100-300 units/mg protein
Synonym: Creatinine Amidohydrolase
CAS Number: 9025-13-2
EC Number: 232-786-8
MDL Number: MFCD00130877
Product Type: Chemical
Figure 1. Stability (powder form); kept under dry conditions
Figure 2. Stability (liquid form); in 50 mM Tris-HCI buffer solution, pH 7.5
Figure 3. pH Activity; 37 °C, 10-min. reaction in 50 mM buffer solution. pH 5.5, acetate; pH 6.0–8.0, phosphate; pH 8.5–9.0, carbonate
Figure 4. Temperature activity; 10-min. reaction in 50 mM K-phosphate buffer, pH 7.4
Figure 5. pH Stability; 25 °C, 16-hr treatment with 50 mM buffer solution: pH 6.0–8.0, phosphate; pH 8.5–9.0, carbonate
Figure 6. Thermal stability; 30-min. treatment with 50 mM phosphate buffer, pH 7.4
| composition | Protein, 65-85% |
| foreign activity | Creatinase and urease ≤1% |
| Hexokinase and ATPase ≤0.1% | |
| form | lyophilized powder |
| mol wt | ~175 kDa |
| Quality Level | 200  |
| specific activity | 100-300 units/mg protein |
| storage temp. | 2-8°C |
| Analysis Note: | Protein determined by biuret. |
| Application: | Creatininase from microorganisms may be used in the preparation of amperometric biosensor by co-immobilization with other enzymes for the determination of creatinine. |
| Application: | This enzyme is useful for enzymatic determination of creatinine when coupled with creatine amidinohydrolase, sarcosine dehydrogenase or sarcosine oxidase and formaldehyde dehydrogenase in clinical analysis. |
| Biochem/physiol Actions: | Creatininase from Pseudomonas sp. is a homohexameric enzyme with a molecular mass of 28.4 kDa per subunit. It is a cyclic amidohydrolase catalysing the reversible conversion of creatinine to creatine. Each monomer contains a binuclear zinc centre near the C termini of the β-strands and the N termini of the main α-helices. These zinc ions indicate the location of the active site. |
| General description: | Isoelectric point: 4.7 Michaelis constants: 3.2 x 10‾2M (Creatinine), 5.7 x 10‾2M (Creatine) Structure: 6 subunits per mol of enzyme (One mol of zinc is bound to each subunit) Inhibitors: Ag+, Hg++, N-bromosuccinimide, EDTA Optimum pH: 6.5 − 7.5 Optimum temp: 70°C pH Stability: pH 7.5 − 9.0 (5°C, 16hr) Thermal stability: Below 70°C (pH 7.5, 30 min) |
| Other Notes: | One unit will hydrolyze 1.0 μmole of creatinine to creatine per min at pH 8.0 and 25 °C |
| Packaging: | 1000 units in glass bottle |
| Physical form: | Lyophilized powder containing sucrose and BSA as stabilizers |
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H334 |
| Precautionary statements | P261 - P284 - P501 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: 100-300 units/mg protein |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 3.5.2.10 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |