Anti-Caveolin-1 antibody produced in rabbit
SIGMA/C3237 - IgG fraction of antiserum, buffered aqueous solution
Synonym: Anti-Caveolin-1; Anti-VIP21
MDL Number: MFCD01633097
Product Type: Chemical
Enhanced Validation-By Independent Antibodies Immunofluorescence. 3T3 cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton X-100. Fixed cells were stained with 1:500 Anti-Caveolin-1 antibody produced in Rabbit (Cat. No. C3237) (B). The antibody developed using Goat Anti-Rabbit IgG, Cy3 Conjugate antibody, and 1:1,000 Monoclonal Anti-Caveolin-1 antibody produced in Mouse, Clone: CAV1 (Cat. No. SAB4200216) (A). The antibody was developed using Goat Anti-Mouse IgG, Atto 448 conjugate antibody. Results Two Anti-Caveolin-1 antibodies, SAB4200216 (A) and C3237 (B), target different regions of Caveolin-1 show similar staining profiles between the two antibodies, demonstrating Independent Antibody Verification.
Immunofluorescence
NIH-3T3 cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton X-100. Fixed cells were stained with 1:500 Rabbit Anti-Caveolin-1 (Cat. No. C3237). The antibody was developed using Goat Anti-Rabbit IgG, Cy3™ conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.
NIH-3T3 cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton X-100. Fixed cells were stained with 1:500 Rabbit Anti-Caveolin-1 (Cat. No. C3237). The antibody was developed using Goat Anti-Rabbit IgG, Cy3™ conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.
Immunoblotting Cell line lysates were separated on SDS-PAGE and probed with 1:2,000 Rabbit Anti-Caveolin-1 (Cat. No. C3237). The antibody was developed using Goat Anti-Rabbit IgG-peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. Rat2; 2. NIH-3T3; 3. A431; 4. HeLa ; 5. C2C12; 6. CHO; 7. MDCK; 8. Rat Lung; 9. Rat Heart
| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| enhanced validation | independent Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous solution |
| mol wt | antigen 22 kDa |
| Quality Level | 200 |
| shipped in | dry ice |
| species reactivity | human, rat, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect immunofluorescence: 1:500 using mouse fibroblast NIH3T3 cell line |
| microarray: suitable | |
| western blot: 1:2,000 using whole cell extract of the human umbilical vein endothelial HUVEC cell line and the rat fibroblast Rat-1 cell line | |
| UniProt accession no. | Q03135  |
| Application: | Anti-Caveolin-1 antibody produced in rabbit has been used in: • indirect immunofluorescence • in confocal microscopy • western blotting |
| Biochem/physiol Actions: | Caveolin family members function as scaffolding proteins to organize and concentrate specific lipids such as cholesterol and glycosylphosphatidylinosi |
| Biochem/physiol Actions: | Caveolin-1 is expected to play a major role in the growth and evolution of cancer. It participates in glucose metabolism. It may also participate in angiogenesis. Cav-1 helps to control the expression of glucose transporters. |
| Biochem/physiol Actions: | The sequence is highly conserved in many species, e.g., identical in rat, mouse, bovine, and dog caveolin-1. The sequence is not found in caveolin-2 or caveolin-3. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Anti-Caveolin-1 is developed in rabbit using a synthetic peptide corresponding to a region at the N-terminus of human caveolin-1 conjugated to keyhole limpet hemocyanin (KLH) as immunogen. Caveolin-1 (CAV1) is a multifunctional scaffolding protein, that has three exons. It is located on chromosome 7q31. |
| General description: | Caveolin, a 20-24-kDa integral transmembrane protein, has been identified as a principal component of caveolae membranes. Caveolin (also termed VIP21) exists in several isoforms termed caveolin-1, caveolin-2 and caveolin-3. Caveolin-1 (20-22 kDa) can exist as two isoforms, caveolin-1α and -1β due to alternative splicing of the mRNA. |
| Immunogen: | synthetic peptide corresponding to a region at the N-terminus of human caveolin-1 (amino acids 2-20). |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |