Calcium chloride dihydrate
SIGMA/C3306 - Molecular Biology, ≥99.0%
Synonym: CaCl2.2H2O; Calcium chloride; Calcium dichloride
CAS Number: 10035-04-8
Empirical Formula (Hill Notation): CaCl2 · 2H2O
Molecular Weight: 147.01
EC Number: 233-140-8
MDL Number: MFCD00149613
Linear Formula: CaCl2 · 2H2O
Product Type: Chemical
| assay | ≥99.0% |
| foreign activity | DNase, RNase, protease, none detected |
| form | powder |
| grade | Molecular Biology |
| InChI | 1S/Ca.2ClH.2H2O/h;2*1H;2* |
| InChI key | LLSDKQJKOVVTOJ-UHFFFAOYSA |
| mp | 176 °C (dec.) (lit.) |
| Quality Level | 200 ![]() |
| SMILES string | O.O.Cl[Ca]Cl |
| solubility | H2O: 294 mg/mL, clear, colorless |
| Application: | Calcium chloride dihydrate has been used: to prepare competent E. coli for transformation(118) in the preparation of calcium polyphosphate nanospheres and to induce alkaline phosphatase(119) as a component of renaturation buffer for the zymogram of Staphylococcus aureus crude enzyme extracts(120) |
| Biochem/physiol Actions: | Calcium chloride is used in the heat-shock transformation of microbes with the plasmid. The positive charged calcium forms a cation bridge with phosphate of DNA and negative charged phosphorylated lipid A in lipopolysaccharide (LPS) aiding the DNA transformation into the microbes. |
| Packaging: | 100, 250, 500 g in poly bottle |
| Symbol | GHS07 |
| Signal word | Warning |
| Hazard statements | H319 |
| Precautionary statements | P305 + P351 + P338 |
| Hazard Codes | Xi |
| Risk Statements | 36 |
| Safety Statements | 22-24 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥99.0% |
| mp | 176 °C (dec.) (lit.) |
| UNSPSC | 12352302 |


