Anti-CIITA antibody, Mouse monoclonal
SIGMA/C3367 - clone 7-1H, purified from hybridoma cell culture
Synonym: Anti-C2TA; Anti-MHC Class II Transactivator
MDL Number: MFCD01864254
Product Type: Chemical
Western Blotting Whole extract of Raji cells were separated on SDS-PAGE and probed with Anti-CIITA antibody, Mouse monoclonal, Clone: 7-1H (Cat. No. C3367). The antibody was developed using 1:10,000 Anti-Mouse IgG (Fab specific)-Peroxidase antibody produced in Goat (Cat. No. A9917). Lanes 1. 4 μg/mL antibody 2. 2 μg/mL antibody 3. 1 μg/mL antibody 4. 0.5 μg/mL antibody 5. Negative Control
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 7-1H, monoclonal |
| concentration | ~2 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen ~130 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | mouse, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: suitable |
| western blot: 2-4 μg/mL using total cell extract of Raji cells | |
| UniProt accession no. | P33076  |
| Application: | The antibody may be used in immunoblotting (∼130 kDa) and immunocytochemistry. |
| Biochem/physiol Actions: | Class II transactivator protein (CIITA) interacts with specific transcription factors and creates the appropriate transcriptional scaffold required for HLA-D gene expression. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Class II transactivator protein (CIITA) protein contains an acidic region in its N-terminal with transactivation activity, that binds to proteins in the basal transcription machinery and to cAMP response element-binding protein (CREB)-binding protein (CBP). The protein contains motifs for nuclear translocation, GTP binding motif and C-terminal leucine-rich repeats. CIITA is expressed constitutively in B lymphocytes and in immature dendritic cells, while in other cells its expression is induced by IFN-γ . |
| General description: | Monoclonal Anti-CIITA (mouse IgG1 isotype) is derived from the hybridoma 7-1H produced by the fusion of mouse myeloma cells (Sp20 cells) and splenocytes from BALB/c mice immunized with purified N-terminal recombinant CIITA, amino acids 1-350. CIITA is expressed constitutively in B lymphocytes and in immature dendritic cells, while in other cells its expression is induced by IFN-γ. Monoclonal Anti-CIITA recognizes human1 and mouse † CIITA. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |