Acetylcholinesterase from Electrophorus electricus (electric eel)
SIGMA/C3389 - Type VI-S, lyophilized powder, 200-1,000 units/mg protein
Synonym: AChE; Acetylcholine acetylhydrolase; Cholinesterase, Acetyl
CAS Number: 9000-81-1
EC Number: 232-559-3
MDL Number: MFCD00081268
Product Type: Chemical
Common enzymatic methods for measuring organophosphate and cholinesterase activity include: colorimetric (using DTNB or ferricyanate to measure thiolesterase products), colorimetric (coupled with choline oxidase and peroxidase), fluorescent, titrimetric (mainly for purified enzyme activity assays). Shown above is the simple uncoupled assay using acetylcholine which can be measured titrimetrically.
Common enzymatic methods for measuring organophosphate and cholinesterase activity include: colorimetric (using DTNB or ferricyanate to measure thiolesterase products), colorimetric (coupled with choline oxidase and peroxidase), fluorescent, titrimetric (mainly for purified enzyme activity assays). Shown above is the coupled assay using choline oxidase and peroxidase.
Common enzymatic methods for measuring organophosphate and cholinesterase activity include: colorimetric (using DTNB or ferricyanate to measure thiolesterase products), colorimetric (coupled with choline oxidase and peroxidase), fluorescent, titrimetric (mainly for purified enzyme activity assays). Shown above is the thiolesterase assay using DTNB detection.
Common enzymatic methods for measuring organophosphate and cholinesterase activity include: colorimetric (using DTNB or ferricyanate to measure thiolesterase products), colorimetric (coupled with choline oxidase and peroxidase), fluorescent, titrimetric (mainly for purified enzyme activity assays). Shown above is the thiolesterase assay using ferricyanate detection.
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: C3389-10KU
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: C3389-2KU
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: C3389-500UN
| application(s) | diagnostic assay manufacturing |
| composition | Protein, ≥45% biuret |
| form | lyophilized powder |
| mol wt | 280 kDa |
| solubility | 20 mM Tris HCl buffer, pH 7.5: soluble 1.0 mg/mL, clear(lit.) |
| specific activity | 200-1,000 units/mg protein |
| storage temp. | −20°C |
| type | Type VI-S |
| Analysis Note: | The activity obtained using acetylcholine as substrate is 30-100 times that obtained with butyrylcholine, using acetylcholinesterase from electric eel. |
| Application: | The enzyme from sigma has been used as a reference to to evaluate the effect of aspartame metabolites on hippocampal acetylcholinesterase activity. The enzyme has also been used in immobilization studies for the rapid detection of acetylthiocholine chloride. |
| Biochem/physiol Actions: | Major degradative enzyme for acetylcholine in vivo. Converts acetylcholine + H2O to choline + acetic acid. |
| General description: | Molecular Weight: 280 kDa Isoelectric Point: 5.5 Extinction Coefficient: E1% = 18.0 (280 nm) Acetylcholinesterase from Electrophorus electricus is a tetramer composed of 4 equal subunits of 70 kDa each. Each subunit contains one active site. The enzyme is a glycoprotein containing hexosamines. |
| Packaging: | 10000 units in poly bottle |
| Packaging: | 500, 2000 units in glass bottle |
| Physical form: | Lyophilized powder containing Tris buffer salts |
| Preparation Note: | This enzyme dissolves in 20 mM Tris-HCl buffer, pH 7.5 at 1 mg/mL concentration, yielding a clear solution. |
| Unit Definition: | One unit will hydrolyze 1.0 μmole of acetylcholine to choline and acetate per min at pH 8.0 at 37 °C. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: 200-1,000 units/mg protein |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 3.1.1.7  ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |