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2′,7′-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein

SIGMA/C3411 - ≥90% (HPLC)

Synonym: BCECF

CAS Number: 85138-49-4
Empirical Formula (Hill Notation): C27H20O11
Molecular Weight: 520.44
MDL Number: MFCD00036970
Linear Formula: C27H20O11
Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-C3411-1MG 1 mg
 $340.00
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assay ≥90% (HPLC)
form powder
InChI 1S/C27H20O11/c28-19-10-21-17(7-12(19)2-5-23(30)31)25(16-9-14(26(34)35)1-4-15(16)27(36)37)18-8-13(3-6-24(32)33)20(29)11-22(18)38-21/h1,4,7-11,28H,2-3,5-6H2,(H,30,31)(H,32,33)(H,34,35)(H,36,37)
InChI key DKROGDOAOXDINY-UHFFFAOYSA-N
Quality Level 100 
SMILES string OC(=O)CCc1cc2c(OC3=CC(=O)C(CCC(O)=O)=CC3=C2c4cc(ccc4C(O)=O)C(O)=O)cc1O
storage temp. −20°C
Application: 2′,7′-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein has been used in a fluorescence-based screening assay, to determine the pH.
Biochem/physiol Actions: 2′,7′-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein (BCECF) is useful to study multidrug resistance-associated protein (MRP) transporter action within the cell. BCECF is known to interfere with drug efflux interactions.
Biochem/physiol Actions: Fluorescent indicator for measurement of cytoplasmic pH in lymphocytes.
RIDADR NONH for all modes of transport
WGK Germany WGK 3
Flash Point(F) Not applicable
Flash Point(C) Not applicable
Purity ≥90% (HPLC)
Storage Temp. −20°C
UNSPSC 12352200

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