Catalase from Aspergillus niger
SIGMA/C3515 - ammonium sulfate suspension, ≥4,000 units/mg protein
Synonym: H2O2:H2O2 oxidoreductase
CAS Number: 9001-05-2
MDL Number: MFCD00081483
Product Type: Chemical
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| biological source | Aspergillus niger |
| form | ammonium sulfate suspension |
| InChI | 1S/C9H10O3/c1-2-12-9(11)7 |
| InChI key | NUVBSKCKDOMJSU-UHFFFAOYSA |
| mol wt | tetramer ~250 kDa |
| Quality Level | 200  |
| shipped in | wet ice |
| SMILES string | O(CC)C(=O)c1ccc(cc1)O |
| specific activity | ≥4,000 units/mg protein |
| storage condition | (Tightly closed) |
| storage temp. | 2-8°C |
| technique(s) | FISH: suitable |
| UniProt accession no. | A2QH56  |
| A2R2G8 |
| Analysis Note: | Protein determined by biuret |
| Application: | Catalase from Aspergillus niger has been used:• as a positive control during the functional characterization of Clostridium difficile spore coat proteins. • as a component of the catalase solution to prepare GLOX buffer with enzymes to maintain the embryos of Caenorhabditis elegans before single-molecule fluorescence in situ hybridization (smFISH) studies • as a component of the imaging buffer for stochastic optical reconstruction microscopy (STORM) imaging of platelet-rich plasma • as a supplement in Todd Hewitt media plus 0.5 % yeast extract (THY) media for the neutralization of pneumococcal H2O2 |
| Biochem/physiol Actions: | Catalase catalyzes the decomposition of hydrogen peroxide into water and oxygen. Each subunit of the tetramer contains iron bound to a protoheme IX group. The enzyme also strongly binds NADP, which is in close proximity to the heme group. Isoelectric point is found to be 6.5. Optimum pH for catalytic activity is 7.0. The enzyme activity is inhibited by 3-amino-1-H-1,2,4 triazole, cyanide, azide, hydroxylamine, cyanogen bromide, 2-mercaptoethanol, dithiothreitol, dianisidine, and nitrate. Incubation of catalase with ascorbate or ascorbate/Cu2+ results in degradation of the catalase molecule. |
| Biochem/physiol Actions: | Catalase is involved in catalyzing the degradation of hydrogen peroxide (H2O2) to water and free oxygen. Catalase is used commercially for decomposing H2O2 as it is added as an antimicrobial agent in process streams. It also catalyzes the oxidation of electron donors like ethanol and phenols during lower concentrations of H2O2. Catalase shows antioxidant activity by protecting cells from higher levels of reactive oxygen species (ROS). Increased expression of catalase is observed in chronic lymphocytic leukemia, gastric cancer, glioma, and melanoma. Decreased levels of catalase are seen in acute myeloid leukemia, lung, pancreatic, prostate, and skin (non-melanoma) cancers. Hence catalase exhibits a dual role in cancer. |
| Disclaimer: | Freezing of solutions is not recommended. |
| General description: | Research area: Cell Signaling Catalase is an active enzyme present in aerobic organisms. It is a ferric hemoprotein and a tetramer. |
| Other Notes: | 2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research |
| Other Notes: | One unit will decompose 1.0 μmole of H2O2 per min at pH 7.0 at 25 °C, while the H2O2 concentration falls from 10.3 to 9.2 mM, measured by the rate of decrease of A240. |
| Packaging: | 10, 25 mg in glass bottle |
| Physical form: | Suspension in 3.2 M (NH4)2SO4 solution, pH 6.0 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥4,000 units/mg protein |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 1.11.1.6 ( BRENDA | IUBMB ) |
| UNSPSC | 12352204 |