Complement C8 from human serum
SIGMA/C3535 - suitable for radioiodination, RIA, ≥85% (SDS-PAGE), ≥125,000 C8H50 units/mg protein (using C8 deficient serum)
CAS Number: 80295-58-5
MDL Number: MFCD00130847
Product Type: Chemical
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| assay | ≥85% (SDS-PAGE) |
| biological source | human |
| concentration | 1 mg/mL in PBS, pH 7.2 |
| form | solution |
| Quality Level | 200  |
| shipped in | dry ice |
| specific activity | ≥125,000 C8H50 units/mg protein (using C8 deficient serum) |
| storage temp. | −70°C |
| suitability | suitable for radioiodination, RIA |
| technique(s) | immunoelectrophoresis: suitable |
| UniProt accession no. | P07357  |
| Analysis Note: | Functionally pure by a sensitive hemolytic assay using depleted sera. |
| Application: | Complement C8 is one of the end terminals of the complement system contained in the membrane attack complex (MAC). In the (MAC), C8 initiates and coordinates MAC pore formation. It has been used in research to investigate the mechanisms of how C8-C9 and C9-C9 interactions facilitate folding of the membrane attack complex perforin (MACPF) domain to form the circular pore. |
| Biochem/physiol Actions: | C8 is one of the components of the terminal complement complex and is required for a functional complement system. It is composed of disulfide-linked C8α and C8γ and a noncovalently associated C8β chain. During formation of the membrane-attack complex, a domain on C8α induces C9 to polymerize and form a pore-like structure. |
| Disclaimer: | RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization. |
| Other Notes: | View more information on the complement pathway at www.sigma-aldrich.com/enz |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥85% (SDS-PAGE) |
| activity | specific activity: ≥125,000 C8H50 units/mg protein (using C8 deficient serum) |
| Storage Temp. | −70°C |
| UNSPSC | 12352202 |