Monoclonal Anti-Calmodulin antibody produced in mouse
SIGMA/C3545 - clone 6D4, ascites fluid
Synonym: Anti-CALM3; Anti-calmodulin
MDL Number: MFCD00162206
Product Type: Chemical
Western Blotting Bovine brain calmodulin preparation were separated on SDS-PAGE and probed with Anti-Calmodulin antibody, Mouse monoclonal, Clone: 6D4 (Cat. No. C3545) followed by 2 μg/mL Goat Anti-Mouse IgG (Fab specific)-Biotin antibody (Cat. No. B7151) and by 2 μg/mL ExtrAvidin®-Peroxidase (Cat. No. E2886). Lanes 1. 1:2,000 antibody 2. Negative Control
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 6D4, monoclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide as preservative |
| isotype | IgG1 |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | chicken, Dictyostelium discoideum, bovine, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | western blot: 1:2,000 using a bovine brain calmodulin preparation |
| UniProt accession no. | P0DP29  |
| Application: | Monoclonal Anti-Calmodulin antibody produced in mouse has been used in: • enzyme-linked immunosorbent assay (ELISA) • immunoblot • dot blot • immunocytochemistry • immunoprecipitation • immunofluorescence |
| Biochem/physiol Actions: | Calmodulin is a ubiquitous molecule which can bind up to 4 calcium ions, regulating and mediating a wide variety of biochemical processes. |
| Biochem/physiol Actions: | The antibody recognizes native and SDS-denatured calmodulin. |
| Biochem/physiol Actions: | These antibodies are directed against different epitopes within the calmodulin molecule. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Calmodulin belongs to a family of structurally homologous Ca2+-binding proteins. CALM1 gene is located on human chromosome 14q32.11. |
| General description: | Calmodulin is a highly conserved 17kDa calcium binding protein found in all eukaryotic cells. It is a multifunctional molecule. Monoclonal anti-Calmodulin (mouse IgG1 isotype) is derived from the 6D4 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with calmodulin, purified from Dictyostelium discoideum and conjugated to KLH. |
| Immunogen: | preparation of purified calmodulin from Dictyostelium discoideum conjugated to KLH. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |