Catalase from human erythrocytes
SIGMA/C3556 - ≥90% (SDS-PAGE), buffered aqueous solution, ≥30,000 units/mg protein
Synonym: H2O2:H2O2 oxidoreductase
CAS Number: 9001-05-2
EC Number: 232-577-1
MDL Number: MFCD00081483
Product Type: Chemical
This image is provided for informative purposes only and does not represent an actual product label. It should not be used as a substitute for official product labeling.
| assay | ≥90% (SDS-PAGE) |
| biological source | human erythrocytes |
| concentration | ≤10 mg/mL |
| form | buffered aqueous solution |
| InChI | 1S/C9H10O3/c1-2-12-9(11)7 |
| InChI key | NUVBSKCKDOMJSU-UHFFFAOYSA |
| mol wt | tetramer ~250 kDa |
| Quality Level | 200  |
| shipped in | wet ice |
| SMILES string | O(CC)C(=O)c1ccc(cc1)O |
| specific activity | ≥30,000 units/mg protein |
| storage temp. | −20°C |
| technique(s) | cell based assay: suitable |
| UniProt accession no. | P04040  |
| Analysis Note: | Protein determined by biuret |
| Application: | Catalase from human erythrocytes has been used: • to prevent reoxidation of reduced cytochrome c by H2O2 while measuring the production of superoxide with cytochrome C. • as a component of the imaging buffer for stochastic optical reconstruction microscopy (STORM) imaging of human skin fibroblasts • as a component of the gloxy mix for single-molecule imaging |
| Biochem/physiol Actions: | Catalase activates the decomposition of hydrogen peroxide, a reactive oxygen species, into water and oxygen. It functions as a natural antioxidant, protecting cells against oxidative damage to proteins, lipids and nucleic acids. Catalase has also been used to study the role reactive oxygen species play in gene expression and apoptosis. |
| Biochem/physiol Actions: | Deficiency of catalase and oxidative stress are associated with vitiligo, hypertension, Alzheimer′s disease, diabetes mellitus, and acatalasemia. Catalase shows a dichotomous role in cancer as its expression is at a higher level in chronic lymphocytic leukemia, glioma, melanoma, and gastric cancer and lower levels of expression are seen in acute myeloid leukemia, pancreatic, lung, prostate, and skin (non-melanoma) cancers. |
| General description: | Research area: Cell Signaling Human catalase is a member of the monofunctional heme-containing catalases. It is an intracellular enzyme located at higher concentrations in the liver, erythrocytes, and kidney. Catalase is a homo-tetrameric protein and comprises amino acid residues, one heme group that is iron III protoporphyrin IX, and a nicotinamide adenine dinucleotide phosphate (NADPH) molecule. It is a ubiquitous enzyme found in most aerobic organisms. The catalase (CAT) gene is located on the human chromosome at 11p13. |
| Other Notes: | One unit will decompose 1.0 μmole of H2O2 per min at pH 7.0 at 25 °C, while the H2O2 conc. falls from 10.3 to 9.2 mM, measured by the rate of decrease of A240. |
| Physical form: | Solution in 50 mM Tris, pH 8.0 |
| Preparation Note: | Tightly closed. Dry. Keep locked up or in an area accessible only to qualified or authorized |
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H334 |
| Precautionary statements | P261 - P284 - P501 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥90% (SDS-PAGE) |
| activity | specific activity: ≥30,000 units/mg protein |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 1.11.1.6 ( BRENDA | IUBMB ) |
| UNSPSC | 12352204 |