Anti-Connexin-32 (106-124) antibody produced in rabbit
SIGMA/C3595 - affinity isolated antibody, buffered aqueous solution
Synonym: Anti-CMTX; Anti-CMTX1; Anti-CX32
MDL Number: MFCD01633266
Product Type: Chemical
Immunohistochemistry Acetone fixed, Rat liver frozen tissue sections were stained with 1:400 Anti-Connexin-32 (106-124) antibody produced in Rabbit (Cat. No. C3595) followed 20 μg/mL Goat Anti-Rabbit IgG (whole molecule)-Biotin (Cat. No. B8895) followed by 20 μg/mL ExtrAvidin®-Peroxidase (Cat. No. E2886).
Immunohistochemistry formalin fixed, paraffin-embedded Rat Kidney tissue sections were stained with 1:400 Anti-Connexin-32 (106-124) antibody produced in Rabbit (Cat. No. C3595) followed 20 μg/mL Goat Anti-Rabbit IgG (whole molecule)-Biotin (Cat. No. B8895) followed by 20 μg/mL ExtrAvidin®-Peroxidase (Cat. No. E2886).
Western Blotting Whole extract of Rat liver were separated on SDS-PAGE and probed with Anti-Connexin-32 (106-124) antibody produced in Rabbit (Cat. No. C3595). The antibody was developed using 1:5,000 Anti-Rabbit IgG (whole molecule)-Alkaline Phosphatase antibody produced in Goat (Cat. No. A9919). Lanes: 1. 1:400 antibody 2. 1:400 antibody + inhibition with immunizing peptide 3. Negative Control
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 27 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | mouse, human, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (frozen sections): 1:400 using frozen rat liver sections |
| western blot: 1:400 using a rat liver membrane preparation | |
| UniProt accession no. | P08034  |
| Application: | Anti-Connexin 32 (265-279) antibody produced in rabbit is suitable for immunohistochemistry (frozen sections) at a dilution of 1:400 using frozen rat liver tissue. It is also suitable for western blot at a dilution of 1:400 using a rat liver membrane preparation. |
| Application: | Anti-Connexin-32 (106-124) antibody produced in rabbit has been used in: • western blotting • immunofluorescence • immunohistology |
| Biochem/physiol Actions: | Cx32 mutations is associated with X-linked Charcot-Marie-Tooth (CMTX) disease, which leads to myelin disruption and axonal degeneration. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Connexins (Cx) are a multi-gene family of highly related proteins with molecular weights ranging from 26 to 70 kDa. The structure of connexin molecules includes a cytoplasmic N-terminal region, four transmembrane domains, two extracellular loops, and a C-terminal cytoplasmic tail of varying length. The 27kD connexin protein (connexin 32, Cx32) is expressed in several tissues. |
| Immunogen: | synthetic human connexin-32 peptide (amino acids 106-124). |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin with 15 mM sodium azide. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |