Anti-Casein Kinase 2β antibody, Mouse monoclonal
SIGMA/C3617 - clone 6D5, purified from hybridoma cell culture
Synonym: Anti-CK2β
MDL Number: MFCD00675215
Product Type: Chemical
Western Blotting Whole extract of Human HepG2 cells were separated on SDS-PAGE and probed with Anti-Casein Kinase 2-β antibody, Mouse Monoclonal, Clone: 6D5 (Cat. No. C3617). The antibody was developed with 1:5,000 Anti-Mouse IgG (Fab specific)-Peroxidase antibody produced in Goat (Cat. No. A2304). Lanes 1. 2 μg/mL antibody 2. 4 μg/mL antibody 3. 8 μg/mL antibody 4. Negative Control
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 6D5, monoclonal |
| concentration | ~2 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen ~30 kDa |
| shipped in | dry ice |
| species reactivity | mouse, hamster, bovine, rat, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | dot blot: suitable |
| indirect ELISA: suitable | |
| microarray: suitable | |
| western blot: 2-4 μg/mL using total cell extract of HepG2 cells | |
| UniProt accession no. | P67870   |
| Application: | Monoclonal Anti-Casein Kinase 2β antibody is suitable for use in indirect immunofluorescence (using A549 cells ). The product can also be used for western blot (2-4 μg/mL using total cell extract of HepG2 cells), microarray, dot blot and indirect ELISA. |
| Biochem/physiol Actions: | Casein Kinase 2β encodes the beta subunit of casein kinase II, a ubiquitous protein kinase which regulates metabolic pathways, signal transduction, transcription, translation, and replication. The enzyme is composed of three subunits, alpha, alpha pr |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Casein Kinase 2 (CK2) is a ubiquitously expressed serine/threonine kinase that has been implicated in various cellular functions such as cell death, cell cycle regulation, and mRNA synthesis. This protein kinase comprises of two catalytic α subunits and two dimeric β subunits that associate to form a heterotetrameric enzyme complex.CK2 is also involved in regulating viral infections Monoclonal Anti-Casein Kinase 2β antibody is specific for human, bovine, rat, mouse and hamster Casein Kinase 2β (approx. 30 kDa). The antibody epitope lies within amino-acids 123-127 of human CK2β. |
| Immunogen: | recombinant human casein kinase 2β (CK2β). The antibody epitope resides within amino acids 123-127. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |