Chondroitinase ABC from Proteus vulgaris

SIGMA/C3667 - BSA free, lyophilized powder, specific activity 50-250 units/mg protein

Synonym: 4.2.2.20; 4.2.2.21; Chondroitin ABC Lyase

CAS Number: 9024-13-9
EC Number: 232-777-9
MDL Number: MFCD00130795
Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-C3667-5UN	5 units	$292.00 1/EA	Add To Favorites
45-C3667-10UN	10 units	$496.00 1/EA	Add To Favorites

Chondroitinase A,B and C specificities

Axonal regeneration inhibitors accumulate at the injury site including a family of inhibitors called Chondroitin sulfate proteoglycans (CSPGs). This family of molecules have a core protein with a sulfated glycosaminoglycan (GAG) side chain. When the GAG is digested by Chondroitinase ABC (ChABC) axonal regeneration can occur.

This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: C3667-10UN

Properties
Descriptions
Safety Info.
Basic Data

application(s)	diagnostic assay manufacturing
biological source	bacterial (Proteus vulgaris)
composition	Protein, ~10% Lowry
conjugate	(Glucosaminoglycan)
foreign activity	protease, essentially free
form	lyophilized powder
mol wt	120 kDa by gel filtration
Quality Level	300
solubility	0.01% bovine serum albumin aqueous (BSA) solution: soluble
specific activity	50-250 units/mg protein
storage temp.	−20°C

Application:	The enzyme has been used to study the consequences of inducing acute, long-lasting changes in chondroitin sulfate proteoglycans using adult rat brain cells. Glycosaminoglycans have been digested with chondroitinase ABC for chondroitin/dermatan sulfate quantitation in keratocyte cell cultures obtained from fresh bovine corneal stromae.
Biochem/physiol Actions:	Chondroitinase ABC catalyzes the eliminative degradation of polysaccharides containing (1-4)-β-D-hexosaminyl and (1-3)-β-D-glucuronosyl or (1-3)-α-L-iduronosyl linkages to disaccharides containing 4-deoxy-β-D-gluc-4-enuronosyl groups. It acts on chondroitin 4-sulfate, chondroitin 6-sulfate, and dermatan sulfate, and acts slowly on hyaluronate. The molecular weight is found to be approximately 120 kDa with 2 non-identical subunits of molecular masses 86 kDa and 32 kDa. The pH optimum is found to be 8.0 with chondroitin sulfate and 6.8 with hyaluronic acid and temperature optimum is 37 °C. 1 mM Zn²⁺ acts as an inhibitor, while 0.05 M acetate is an activator of the enzyme.
Other Notes:	Contains potassium phosphate buffer salts and stabilizer
Other Notes:	One unit will liberate 1.0 μmole of a mixture of 2-acetamido-2-deoxy-3-O-(β-D-gluc-4-ene-pyranosyluronic acid)-4-O-sulfo-D-galactose and 1.0 μmole of 2-acetamido-2-deoxy-3-O-(β-D-gluc-4-ene-pyranosyluronic acid)-6-O-sulfo-D-galactose from chondroitin sulfate from shark cartilage, per min at pH 8.0 at 37 °C.
Other Notes:	View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer
Packaging:	5, 10 units in glass bottle
Packaging:	Packages based on chondroitinase C activity.
Preparation Note:	Affinity purified

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

activity	specific activity: 50-250 units/mg protein
Storage Temp.	−20°C
Enzyme Commission (EC) Number	4.2.2.4 ( BRENDA \| IUBMB )
UNSPSC	12352204

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