Cytochrome P450 human
SIGMA/C3735 - 1A1 Isozyme Microsomes, with P450 Reductase, recombinant, expressed in baculovirus infected insect cells (BTI-TN-5B1-4)
MDL Number: MFCD03456520
Product Type: Chemical
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| application(s) | cell analysis |
| biological source | human |
| form | solution |
| mol wt | 45-60 kDa |
| packaging | vial of 0.5 nmol |
| Quality Level | 200  |
| recombinant | expressed in baculovirus infected insect cells (BTI-TN-5B1-4) |
| shipped in | dry ice |
| solubility | water: soluble |
| specific activity | ≥4 units/pmol enzyme |
| storage temp. | −70°C |
| suitability | suitable for molecular biology |
| technique(s) | activity assay: suitable |
| UniProt accession no. | P04798  |
| Biochem/physiol Actions: | Cytochrome P450 is a heterogeneous family of isozymes whose primary function is to oxidize small molecules, both as a function of intermediary metabolism (e.g., fatty acids) and to detoxify exogenous compounds (drugs or toxins). Some isoforms have narrow substrate specificity, while others are promiscuous. The CYP1A1 isoform catalyzes 7-deethylation of ethoxyresorufin. Cytochrome P450 (CYP) plays an important role in detoxifying xenobiotics, cellular metabolism and homeostasis. One of the main mechanisms of drug-drug interactions is the induction or inhibition of these enzymes. CYP enzymes are transcriptionally activated by a variety of xenobiotics and by endogenous substrates via receptor-dependent pathways. Inhibition of these enzymes is a major factor in metabolism-based drug-drug interactions, and many chemotherapeutic medications can cause drug interactions by either inhibiting or inducing the cytochrome p450 enzyme system. |
| General description: | Research area: Immuno and CKS Cytochrome P450 (CYP) enzymes are a family of enzymes that are encoded by the P450 genes. These enzymes are membrane bound hemoproteins. that are mainly found in the liver’s endoplasmic reticulum. |
| Physical form: | Solution in 100 mM potassium phosphate buffer, pH 7.4. |
| Preparation Note: | Microsomes containing human CYP1A1 and recombinant human NADPH-P450 reductase. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥4 units/pmol enzyme |
| Storage Temp. | −70°C |
| Enzyme Commission (EC) Number | 1.6.2.4 ( BRENDA | IUBMB ) |
| UNSPSC | 12161501 |