Anti-N-Cadherin antibody, Mouse monoclonal
SIGMA/C3865 - clone GC-4, purified from hybridoma cell culture
Synonym: Anti-A-CAM; Anti-A-Cell Adhesion Molecule
MDL Number: MFCD00164511
Product Type: Chemical
Immunohistochemistry Frozen acetone fixed chicken cardiac sections stained with 10 μg/mL Monoclonal Anti-N-Cadherin antibody produced in Mouse clone GC-4: (Cat. No. C3865) followed by Anti-Mouse IgG (Fab specific)-FITC antibody produced in Goat (Cat. No. F5262).
Immunofluorescence HeLa cells were fixed and permeabilized with methanol followed by methanol:acetone. Fixed cells were stained with 20 μg/mL Monoclonal Anti-N-Cadherin, Clone: GC-4 (Cat. No. C3865). The antibody was developed using Goat Anti-Mouse IgG, Cy3 conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.
Western Blotting COS-7 cells lysates were separated on SDS-PAGE and probed with Monoclonal Anti-N-Cadherin antibody produced in Mouse, Clone: GC-4 (Cat. No. C3865). The antibody was developed using 1:4,000 Anti-Mouse IgG (Fab specific)-Peroxidase antibody produced in Goat (Cat. No. A9917). Lanes 1. 10 μg/mL antibody 2. 20 μg/mL antibody 3. Negative Control
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| application(s) | research pathology |
| biological source | mouse |
| clone | GC-4, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen 135 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | chicken, monkey, mouse, human, rabbit, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | electron microscopy: suitable |
| flow cytometry: suitable | |
| immunocytochemistry: suitable | |
| immunohistochemistry: 10-20 μg/mL using rat cardiac muscle frozen sections | |
| western blot: 10-20 μg/mL using chicken cardiac muscle or COS-7 cell extracts | |
| UniProt accession no. | P19022  |
| Application: | Anti-N-Cadherin antibody is suitable for the following procedures: • electron microscopy • flow cytometry • immunocytochemistry • immunohistochemistry at a concentration of 10-20μg/mL using rat cardiac muscle frozen sections • western blot at a concentration of 10-20μg/mL using chicken cardiac muscle or COS-7 cell extracts |
| Biochem/physiol Actions: | CDH11 gene encodes a type II classical cadherin that mediate calcium-dependent cell-cell adhesion. |
| Biochem/physiol Actions: | Higher expression of CDH2 gene was observed in tumor cells. It is known to induce the invasive and chemoresistant attributes to the cancer cells. N-glycosylation of N-cadherin is associated with the process of tumorigenesis especially in mammary gland, renal system, and ovary. Loss of expression of the gene was observed in adrenocortical carcinoma. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | N-Cadherin is a member of the cadherin family of proteins that mediate cell-cell adhesion in a calcium dependent manner. N-cadherin is a transmembrane glycoprotein that localizes in the cell-cell adherens junction on the plasma membrane. The protein is known to be localized in the cell membrane. |
| Other Notes: | Clone GC-4 is also known as GB-9. |
| Packaging: | 200 μL in glass bottle |
| Physical form: | 0.2 μm-filtered solution in 0.01 M phosphate buffered saline, pH 7.4. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |