Anti-Calsequestrin-2 antibody produced in rabbit
SIGMA/C3868 - ~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution
Synonym: Anti-CASQ2; Anti-fast-twitch, cardiac muscle Calsequestrin
Product Type: Chemical
Enhanced Validation-By Recombinant Expression Antibody recognition: Anti-Calsequestrin-2 antibody produced in Rabbit (Cat. No. C3868) is confirmed by western blot in the cell sample that has been altered to overexpress the target protein-HEK-293T over expressing Calsequestrin-2 (Lane 1) while no staining is detected in the wild type sample-HEK-293T (Lane 2). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and a chemiluminescent substrate. Antibody dilution: 1:2,000.
Immunoblotting Tissue extracts and whole cell lysates were separated on SDS-PAGE and blotted with Anti-Calsequestrin-2 (Cat. No. C3868). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and chemiluminescent substrate. Antibody dilution 1:2,000 Lanes 1,5. rat heart (S1 fraction) 2. HEK293 cells transfected with human calsequestrin-2 3. HEK293 cells (untransfected) 4. rat skeletal muscle 5. Inhibition with calsequestrin-2 immunizing peptide (mouse 57-74) (10 μg/mL)
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous solution |
| mol wt | antigen ~55 kDa |
| Quality Level | 200 |
| shipped in | dry ice |
| species reactivity | rat, human |
| species reactivity (predicted by homology) | mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | western blot: 0.5-1.0 μg/mL using extract of rat cardiac muscle (S1 fraction) |
| western blot: 0.5-1.0 μg/mL using HEK-293T cells expressing human calsequestrin-2 | |
| UniProt accession no. | O14958  |
| Application: | Anti-Calsequestrin-2 antibody has been used in immunoblotting, western blotting. |
| Application: | Anti-Calsequestrin-2 is suitable for western blot at a concentration of 0.5-1.0μg/mL using HEK-293T cells expressing human calsequestrin-2 and western blot at a concentration of 0.5-1.0μg/mL using extract of rat cardiac muscle (S1 fraction) |
| Biochem/physiol Actions: | Over expression of cardiac calsequestrin (CASQ2) is associated with depressed cardiovascular function and hypertrophy. Mutations in CASQ2 are thought to be associated with catecholaminergic polymorphic ventricular tachycardia (CPVT). It is a high-capacity, low-affinity Ca2+ binding glycoprotein, which functions as an internal Ca2+ store in the lumen of the sarcoplasmic reticulum (SR). |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Calsequestrin is a major Ca2+ binding protein in cardiac and skeletal muscle. |
| General description: | Calsequestrin-2 (55 kDa) is found in both sarcoplasmic reticulum′s (SR) terminal cisternae luminal space of both cardiac and in slow skeletal muscle cells. It is made of three domains each with negatively charged thioredoxin-like folds with a β-sheet surrounded by four α-helices. |
| Immunogen: | synthetic peptide corresponding to amino acids 57-74 of rat calsequestrin-2, conjugated to KLH. This sequence is highly conserved in human and rat calsequestrin-2 (single amino acid substitution). This sequence is not found in calsequestrin-1. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |