Carboxypeptidase Y from baker’s yeast (S. cerevisiae)
SIGMA/C3888 - lyophilized powder, ≥50 units/mg protein
Synonym: Peptidyl-L-amino acid Hydrolase; Serine Carboxypeptidase
CAS Number: 9046-67-7
MDL Number: MFCD00130723
Product Type: Chemical
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| composition | Protein, ≥75% E |
| form | lyophilized powder |
| grade | Proteomics Grade |
| mol wt | 61 kDa |
| Quality Level | 200  |
| shipped in | wet ice |
| specific activity | ≥50 units/mg protein |
| storage temp. | −20°C |
| Analysis Note: | amidase and esterase activities may be present |
| Application: | Carboxypeptidase Y has a broad specificity and is stable in urea. Hence, this enzyme is not like other carboxypeptidases and can be used for sequence analysis. Due to its amidase action, this enzyme might be applied to the sequence analysis of peptides having amidated COOH-terminal groups such as oxytocin and vasopressin. |
| Biochem/physiol Actions: | The glycoprotein has a molecular weight of about 61,000, has a nitrogen content of 12.74%. It is a single polypeptide chain of 442 residues with 16 residues of glucosamine in the carbohydrate moiety. Lysine is at the NH2 terminus and -Asp-Ser-Thr-Leu is the COOH-terminal sequence. The principal action of the enzyme is to remove COOH-terminal residues from polypeptide chains. It is used as a vacuolar marker enzyme for studies on protein transport and localization. |
| Other Notes: | One unit will hydrolyze 1.0 μmole of N-CBZ-Phe-Ala to N-CBZ- |
| Packaging: | 1, 5 mg in glass bottle |
| Packaging: | Package size based on protein content |
| Physical form: | Lyophilized powder containing citric acid. |
| Symbol |   GHS07,GHS08 |
| Signal word | Danger |
| Hazard statements | H315 - H319 - H334 - H335 |
| Precautionary statements | P302 + P352 - P305 + P351 + P338 |
| Hazard Codes | Xn |
| Risk Statements | 36/37/38-42 |
| Safety Statements | 22-26-45 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥50 units/mg protein |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 3.4.16.1 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |