Cyanoborohydride Coupling Buffer
SIGMA/C4187
Synonym: Coupling buffer
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: C4187-1L.
| application(s) | life science and biopharma |
| form | liquid |
| Quality Level | 200  |
| reaction suitability | reagent type: reductant |
| storage temp. | 2-8°C |
| technique(s) | affinity chromatography: suitable |
| Application: | A ready-to-use reagent used to couple amine ligands to aldehyde functional groups. The coupling buffer reaction is a reductive amination of the intermediate Schiff′s base to a stable C−N bond. |
| Application: | Cyanoborohydride Coupling Buffer has been used: • in coupling reactions between amines and glutaraldehyde • to reduce hydrazone bond to a stable hydrazide bond • as a component in |
| Application: | Cyanoborohydride Coupling Buffer is used in affinity chromatography, protein chromatography, activated/functionalized matrices and synthetic reagents. Cyanoborohydride has been used to inform a safe and effective gene-transfer system targeting hepatocytes as well as to develop a method for targeted delivery of anticancer therapeutics to cancer cells in hypoxic areas. |
| Biochem/physiol Actions: | Cyanoborohydride Coupling Buffer is a reagent suitable for reductive amination processes, that contributes to transformation of simple alcohols into more complex amines. It is used in the conversion of Schiff base, by reducing it, to form a secondary amine without affecting aldehyde groups on the support. |
| Preparation Note: | 0.02 M sodium phosphate, pH 7.5, containing 0.2 M sodium chloride and 3.0 g/L sodium cyanoborohydride |
| Symbol |   GHS07,GHS09 |
| Signal word | Warning |
| Hazard statements | H302 - H411 |
| Precautionary statements | P264 - P270 - P273 - P301 + P312 - P391 - P501 |
| Hazard Codes | Xn,N |
| Risk Statements | 20/21/22-51/53 |
| Safety Statements | 36/37-61 |
| RIDADR | UN 3082 9 / PGIII |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12161700 |