CelLytic™ Y Cell Lysis Reagent
SIGMA/C4482 - For yeast cells
Synonym: Yeast cell lysis reagent
Product Type: Chemical
Figure 1. CelLytic Y vs. Glass Beads Method.
One gram of S. cerevisiea washed cell pellet was extracted with the following: CelLytic Y, a competitor’s reagent, or glass beads procedure. Protein was determined using BCA protein assay.
One gram of S. cerevisiea washed cell pellet was extracted with the following: CelLytic Y, a competitor’s reagent, or glass beads procedure. Protein was determined using BCA protein assay.
Figure 2. DTT Supplementation.
One gram S. cerevisiae washed cell pellet was extracted with CelLytic Y with or without 5 mM DTT. Protein was determined using BCA protein assay.
One gram S. cerevisiae washed cell pellet was extracted with CelLytic Y with or without 5 mM DTT. Protein was determined using BCA protein assay.
| form | solution |
| Quality Level | 200  |
| Application: | CelLytic™ Y Cell Lysis Reagent has been used in lysis of yeast cells expressing DNA repair, Rad protein, heat shock protein (ssa1) and human interleukin 4 (hIL4). |
| Features and Benefits: | • Fast: Cell lysis in just 20 minutes • Efficient: Three times more efficient than glass beads • Flexible: Lysis is enhanced by the addition of reducing reagent (DTT) • Gentle: Non-denaturing - preserves biological activity |
| General description: | CelLytic Y Cell Lysis Reagent is versatile, phosphate-free and non-denaturing. It provides an effective method for cell lysis and protein solubilization. Target proteins maintain immunoreactivity or biological function. The protocol is brief and preformed at room temperature. No extreme conditions or glass beads are required. Use only 2.5-5 ml/gram yeast cells. |
| Legal Information: | CelLytic is a trademark of Sigma-Aldrich Co. LLC |
| Hazard Codes | Xi |
| Risk Statements | 36 |
| Safety Statements | 26 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| UNSPSC | 41116134 |