Monoclonal Anti-Caldesmon (Smooth) antibody produced in mouse
SIGMA/C4562 - clone hHCD, ascites fluid
Synonym: Anti-CDM; Anti-H-CAD; Anti-HCAD; Anti-L-CAD; Anti-LCAD; Anti-NAG22; Anti-h-CD
MDL Number: MFCD00164554
Product Type: Chemical
Immunohistochemistry Formalin fixed, paraffin-embedded Human Tonsil with Skeletal Muscle tissue sections were stained with 1:500 Monoclonal Anti-Caldesmon (Smooth) antibody produced in Mouse, Clone: hHCD (Cat. No. C4562), followed by 20 μg/mL Anti-Mouse IgG (Fab specific)-Biotin antibody produced in Goat (Cat. No. B7151) and by 20 μg/mL ExtrAvidin®-Peroxidase (Cat. No. E2886).
Immunohistochemistry Formalin fixed, paraffin-embedded Human Tonsil with Skeletal Muscle tissue sections were stained with 1:500 Monoclonal Anti-Caldesmon (Smooth) antibody produced in Mouse, Clone: hHCD (Cat. No. C4562), followed by 20 μg/mL Anti-Mouse IgG (Fab specific)-Biotin antibody produced in Goat (Cat. No. B7151) and by 20μg/mL ExtrAvidin®-Peroxidase (Cat. No. E2886).
Western Blotting Whole extract of Human Uterus were separated on SDS-PAGE Monoclonal and probed with Anti-Caldesmon (Smooth) antibody produced in Mouse, Clone: hHCD (Cat. No. C4562), followed by 1:10,000 Anti-Mouse IgG (Fab specific)-Peroxidase antibody produced in Goat (Cat. No. A9917). Lanes 1. 1:4,000 antibody 2. Negative Control
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | hHCD, monoclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| isotype | IgG1 |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | sheep, rabbit, human, pig, bovine, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: 1:500 using methacarn-fixed, paraffin-embedded sections of human or animal tissue |
| immunoprecipitation (IP): suitable | |
| western blot: 1:4,000 using human uterus extract | |
| UniProt accession no. | Q05682  |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Immunohistochemistry (1 paper) Western Blotting (1 paper) |
| Application: | Monoclonal Anti-Caldesmon (Smooth) antibody produced in mouse is suitable for: • immunohistochemistry at a dilution of 1:500 using methacarn-fixed, paraffin-embedded sections of human or animal tissue • immunoprecipitation • western blot at a dilution of 1:4,000 using human uterus extract |
| Biochem/physiol Actions: | Monoclonal Anti-Caldesmon (Smooth) (mouse IgG1 isotype) reacts in immunoblotting assays with caldesmon polypeptide of 150 kDa (h-Caldesmon). |
| Biochem/physiol Actions: | The antibody (also cited as h-CD) reacts in immunoblotting assays with caldesmon polypeptide of 150 kDa (h-caldesmon). It does not cross-react with skeletal or cardiac muscle or with the 70 kDa non-muscle caldesmon. In immunohistochemical staining, the antibody exhibits smooth muscle specificity. It stains vascular and visceral smooth muscle cells but not epithelial, endothelial or connective tissue fibroblast cells. In normal and malignant breast tissue, the myoepithelial component of galactophorous sinuses (but not in ducts of lobules) is stained; however, the antibody may be non-reactive by immunocytochemical methods with cultured smooth muscle cells. This probably reflects the down regulation of caldesmon commonly observed in tissue culture. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Immunogen: | human uterus smooth muscle extract. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |