Anti-β-Tubulin−Cy3 antibody, Mouse monoclonal
SIGMA/C4585 - clone TUB 2.1, purified from hybridoma cell culture
Synonym: Monoclonal Anti-β-Tubulin
Product Type: Chemical
Immunofluorescence Chicken fibroblasts cells were fixed and permeabilized with cold methanol followed by cold acetone. Fixed cells were stained with 1:200 Monoclonal Anti-β-Tubulin-Cy3 antibody Clone: TUB-2.1 (Cat. No. C4585).
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | TUB 2.1, monoclonal |
| conjugate | CY3 conjugate |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen 55 kDa |
| Quality Level | 200  |
| shipped in | wet ice |
| species reactivity | human, rat, frog, moth, mouse, plant, rabbit, chicken, bovine, wheat, sea urchin, hamster |
| storage condition | protect from light |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | direct immunofluorescence: 1:100 using cultured chicken fibroblasts or BHK cells |
| Application: | Monoclonal Anti-β-Tubulin-Cy3 antibody produced in mouse has been used in: • immunohistochemical assays • immunofluorescence • western blotting • immunocytochemistry • immunoblotting • immunostaining |
| Biochem/physiol Actions: | Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement and in the cytoskeleton. Mutation in the β-tubulin gene leads to various neuronal migration disorders such as lissencephaly, pachygyria and polymicrogyria malformations. |
| Biochem/physiol Actions: | The antibody recognizes all five isoforms of β-tubulin (β1-β5). It reacts with the β-Lc and β-Sc fragments in the carboxy-terminal part of β−tubulin in immunoblotting. The antibody may be used to localize β−tubulin in cultured cells or tissue sections. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-b-Tubulin (mouse IgG1 isotype) is derived from the TUB2.1 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from mouse immunized with purified rat brain tubulin. Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Tubulin is a heterodimer, which consists of α-tubulin and β-tubulin; both subunits have a molecular weight of 55 kDa and share considerable homology. β-tubulin structure is characterized with the core of two β-sheets enclosed by α-helices. |
| Immunogen: | tubulin from rat brain. |
| Legal Information: | Cy is distributed under license from Amersham Biosciences Limited. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |