Anti-Calreticulin antibody produced in rabbit
SIGMA/C4606 - IgG fraction of antiserum, buffered aqueous solution
MDL Number: MFCD01092536
Product Type: Chemical
Immunocytochemistry-immunoflourescence JOURNAL CITATION: Early involvement of cellular stress and inflammatory signals in the pathogenesis of tubulointerstitial kidney disease due to UMOD mutations. By: Trudu, M., Schaeffer, C., et al. in Sci Rep, 2017. PubMed ID: 28785050 Image collected and cropped by CiteAb from the following publication, (http://www.nature.com/articles/s41598-017-07804-6), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunofluorescence MDCK cells were fixed and permeabilized with 3%-4% paraformaldehyde followed by 0.5% Triton™ X-100. Fixed cells were stained with 1:100 Anti-Calreticulin antibody produced in Rabbit (Cat. No. C4606). The antibody was developed using 1:100 Anti-Rabbit IgG (whole molecule)-FITC antibody produced in Goat (Cat. No. F9887).
Immunoblotting HeLa cells (human) were separated on SDS-PAGE and probed with IgG Fraction AB to Calreticulin (RB) (Cat. No. C4606). The antibody was developed with Alk Phos APA Rabbit IgG (gt) (Cat. No. A9919) and a NBT/BCIP substrate. Antibody dilution 1/4,000.
| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 50 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human, canine |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): suitable using whole cell RIPA lysate of the human epitheloid carcinoma HeLa cell line |
| indirect immunofluorescence: 1:100 using 3% paraformaldehyde-fixed, 0.5% Triton X-100 treated, Madin Darby canine kidney MDCK cell line | |
| microarray: suitable | |
| western blot: 1:2,000 using whole cell RIPA lysate of the human epitheloid carcinoma HeLa cell line | |
| UniProt accession no. | P27797  |
| Application: | Anti-Calreticulin antibody produced in rabbit has been used in: • immunoblotting • immunofluorescence • immunocytochemistry{91 • immunoprecipitation |
| Biochem/physiol Actions: | Anti-Calreticulin recognizes human and dog calreticulin (55-60 kDa). |
| Biochem/physiol Actions: | Calreticulin facilitates transient interaction with newly synthesized cellular and extracellular proteins for folding and assembling in the endoplasmic reticulum (ER) before its localization to the cytosol or cell surface. Calreticulin acts as a lectin-like chaperone binding oligosaccharide residues of newly synthesized N-linked glycoproteins and misfolded proteins. It is believed to play a critical role in quality control processes during protein synthesis and folding and calcium (Ca2+) homeostasis. Increased expression of calreticulin increases the Ca2+ storage capacity of the ER. It also appears to modulate store-operated Ca2+-influx and to alter Ca2+ transport by the sarcoplasmic/ER Ca2+-adenosine triphosphatase (ATPase) (SERCA). Overexpression of calreticulin results in increased sensitivity of HeLa cells to drug-induced apoptosis. However, increased resistance to apoptosis has been observed in calreticulin-deficient cells. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | The gene for calreticulin (CALR) is located on the human chromosome 19p13.13. The encoded protein is a predominantly conserved calcium-binding chaperone, which is localized primarily to the lumen of the endoplasmic reticulum (ER). Calreticulin comprises three domains, a globular N-domain, a proline-rich P-domain, and a highly acidic C-domain. |
| Immunogen: | synthetic peptide corresponding to the C-terminus of human calreticulin (amino acids 401-417). |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Preparation Note: | Store at -20 °C. For continuous use, the product maybe stored at 2-8 °C for up to one month. For prolonged storage, freeze in working aliquots at-20 °C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded ifnot used within 12 hours. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |