Monoclonal Anti-Cyclin A antibody produced in mouse
SIGMA/C4710 - clone CY-A1, ascites fluid
MDL Number: MFCD00243105
Product Type: Chemical
Western Blotting JOURNAL CITATION: Lysosomal activity maintains glycolysis and cyclin E1 expression by mediating Ad4BP/SF-1 stability for proper steroidogenic cell growth. By: Syu, J. S., Baba, T., et al. in Sci Rep, 2017. PubMed ID: 28325912 Image collected and cropped by CiteAb from the following publication, (http://www.nature.com/articles/s41598-017-00393-4), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Western Blotting JOURNAL CITATION: Lysosomal activity maintains glycolysis and cyclin E1 expression by mediating Ad4BP/SF-1 stability for proper steroidogenic cell growth. By: Syu, J. S., Baba, T., et al. in Sci Rep, 2017. PubMed ID: 28325912 Image collected and cropped by CiteAb from the following publication, (http://www.nature.com/articles/s41598-017-00393-4), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunofluorescence HeLa cells were fixed and permeabilized with cold methanol followed by cold methanol:acetone. Fixed cells were stained with 1:200 Monoclonal Anti-Cyclin A Clone: CY-A1 (Cat. No. C4710). The antibody was developed using Goat Anti-Mouse IgG, Cy3 conjugate.
Immunoblotting
Cell line lysates were separated on SDS-PAGE and probed with 1:1,000 Monoclonal Anti-Cyclin A Clone: CY-A1 (Cat. No. C4710). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate.
Lanes
1. HeLa
2. K562
3. NCI-H1299
4. HEK-293T
5. Caco-2
6. LA4
7. NIH-3T3
8. PC-12
Cell line lysates were separated on SDS-PAGE and probed with 1:1,000 Monoclonal Anti-Cyclin A Clone: CY-A1 (Cat. No. C4710). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate.
Lanes
1. HeLa
2. K562
3. NCI-H1299
4. HEK-293T
5. Caco-2
6. LA4
7. NIH-3T3
8. PC-12
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | CY-A1, monoclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| isotype | IgG2a |
| mol wt | antigen 60 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | mouse, human, monkey, bovine |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: suitable |
| microarray: suitable | |
| western blot: 1:1,000 using a mouse NIH 3T3 cell extract | |
| UniProt accession no. | P20248  |
| Application: | Monoclonal Anti-Cyclin A antibody produced in mouse is suitable for: • immunocytochemistry • microarray • western blotting at a dilution of 1:1,000 using a mouse NIH 3T3 cell extract |
| Biochem/physiol Actions: | The antibody recognizes an epitope present in amino acids 162-433 of bovine cyclin A. No cross-reactivity is detected with other cyclins (cyclin B, D, or E). |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Cyclin A is a nuclear protein during S phase and it binds both cdk2 and cdc2. The availability of monoclonal antibody reacting specifically with cyclin A enables the subcellular detection and localization of cyclin A and the measurement of relative differences in cyclin A levels as a function of cell cycle phase. |
| Immunogen: | recombinant bovine cyclin A. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |