Anti-Calnexin antibody produced in rabbit
SIGMA/C4731 - IgG fraction of antiserum, buffered aqueous solution
Synonym: Anti-CNX; Anti-IP90; Anti-P90
MDL Number: MFCD01094399
Product Type: Chemical
Immunofluorescence MDCK Madin Darby Canine Kidney cells were fixed and permeabilized with methanol followed by acetone. Fixed cells were stained with 1:200 Anti-Calnexin antibody produced in Rabbit (Cat. No. C4731) followed by 1:100 Anti-Rabbit IgG (whole molecule)-FITC antibody produced in Goat (Cat. No. F9887). Picture A: cells were counterstained with DAPI (blue) to stain nuclei.
Enhanced Validation-By Independent Antibodies Immunofluoresence. HeLa cells were fixed and permeabilized with methanol followed by acetone. Fixed cells were stained with 1:200 Anti-Calnexin antibody produced in Rabbit (Cat. No. C4731) (A). The antibody developed using Goat Anti-Rabbit IgG, FITC Conjugate antibody, and 1:400 Anti-Calnexin antibody, Mouse Monoclonal, Clone: TO-5 (Cat. No. C7617) (B). The antibody was developed using Goat Anti-Mouse IgG, Cy3 Conjugate antibody. Results Two Anti Calnexin antibodies, C4731 (A) and C7617 (B) target different regions of Calnexin show similar staining profiles between the two antibodies, demonstrating Independent Antibody Verification.
Immunoblotting HeLa cells (human) extract was separated on SDS-PAGE and probed with IgG Fraction AB to Calnexin (RB) (Cat. No. C4731). The antibody was developed with Alk Phos APA Rabbit IgG (gt) (Cat. No. A9919) and a NBT/BCIP substrate. Antibody dilution 1/2,000.
| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| enhanced validation | independent Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous solution |
| mol wt | antigen 90 kDa |
| packaging | antibody small pack of 25 μL |
| Quality Level | 200 |
| shipped in | dry ice |
| species reactivity | canine, rat, mouse, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): 1:2,000 using whole cell RIPA lysate of the human epitheloid carcinoma HeLa cell line |
| indirect immunofluorescence: 1:200 using 3% paraformaldehyde-fixed, 0.5% Triton X-100 treated, Madin Darby canine kidney MDCK cell line | |
| microarray: suitable | |
| western blot: 1:2,000 using whole cell RIPA lysate of the human hepatocytoma HepG2 cell line | |
| UniProt accession no. | P27824  |
| Application: | Anti-Calnexin antibody produced in rabbit has been used in: • western blot analysis • immunofluorescence • dual immunofluorescence staining |
| Biochem/physiol Actions: | Calnexin binds newly synthesized glycoproteins and misfolded proteins and is believed to play a critical role in quality control processes during protein synthesis and folding. Calnexin acts as a lectin-like chaperone that binds oligosaccharide residues of newly synthesized N-linked glycoproteins. Calnexin has been shown to be associated with several cell surface proteins, including major histocompatibility complex (MHC) class I heavy chain, T-cell receptor (TCR), and B cell membrane immunoglobulin during translocation through the endoplasmic reticulum (ER). It also forms complexes with other resident ER proteins involved in Ca2+ -dependent retention of proteins. |
| Biochem/physiol Actions: | Rabbit anti-calnexin antibody reacts specifically with calnexin (90 kDa) of dog, mouse, rat and human. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Calnexin contains a long (461 amino acids) N-terminal Ca2+ -binding domain extending into the lumen of the endoplasmic reticulum (ER), a short (22 amino acids) transmembrane segment and an acidic cytosolic domain (96 amino acids). |
| General description: | Calnexin, is a calcium binding type I integral membrane protein present mainly in the endoplasmic reticulum. It plays a pivotal role in quality control processes during protein synthesis and folding. Anti-calnexin antibody (diluted 1: 300) can be used in dual immunofluorescence staining to visualize TG2 transport. |
| Immunogen: | Synthetic peptide corresponding to the c-terminus of human calnexin (amino acids 573-592) conjugated to KLH. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |