α-Chymotrypsinogen A from bovine pancreas
SIGMA/C4879 - essentially salt-free, lyophilized powder
Synonym: chymotrypsin A zymogen
CAS Number: 9035-75-0
EC Number: 232-905-3
MDL Number: MFCD00130483
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: C4879-5G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: C4879-100MG
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: C4879-250MG
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: C4879-1G
| biological source | bovine pancreas |
| foreign activity | α-chymotrypsin ≤1 U/mg (prior to activation by trypsin) |
| form | essentially salt-free, lyophilized powder |
| mol wt | 25,656 Da by calculation |
| purified by | 6× crystallization |
| Quality Level | 200  |
| solubility | 1 mM HCl: soluble 10 mg/mL, clear, colorless |
| specific activity | ≥40 units/mg solid |
| storage temp. | −20°C |
| type | Type II |
| UniProt accession no. | P00767  |
| Application: | The enzyme from Sigma has been used in the non-invasive determination of solid-state protein conformation using near infrared (NIR) spectroscopy. It has been used to study the partitioning of protein in polymer/polymer aqueous two-phase systems. The enzyme has also been used for self-interaction chromatography applications, to test the rapid measurement of protein osmotic second virial coefficients. In this technique, the protein is immobilized on chromatographic particles and its retention is measured using isocratic elution. |
| Application: | α-Chymotrypsinogen A from bovine pancreas has been used as model protein crystallization reproducibility studies. It has also been used in the hydrolysis of α-gliadins prior to mass spectroscopy studies. |
| Biochem/physiol Actions: | A serine protease that hydrolyzes peptide bonds with aromatic or large hydrophobic side chains (Tyr, Trp, Phe, Met) on the carboxyl end of the peptide bond. |
| Biochem/physiol Actions: | Chymotrypsinogen A requires limited proteolysis for its activation. Chymotrypsinogen A may be activated by trypsin and chymotrypsin (autolytic activation) to form m α, β, γ, δ and π chymotrypsin (depending upon the conditions of activation). Chymotrypsin is a protease that will preferentially cleave peptides on the carboxyl side of aromatic amino acids including tryptophan, tyrosine, and phenylalanine. It will also hydrolyze peptides on the carboxyl side of leucine, methionine, and alanine. |
| General description: | Chymotrypsinogen from bovine pancreas is a zymogen containing 5 disulfide bridges. It has an isoelectric pH of 8.97. |
| Other Notes: | After activation to Chymotrypsin, one unit will hydrolyze 1.0 μmole of BTEE per min at pH 7.8 at 25 °C. |
| Other Notes: | View more information on chymotrypsin at www.sigma-aldrich.com/enz |
| Packaging: | 1, 5 g in poly bottle |
| Packaging: | 100, 250 mg in poly bottle |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥40 units/mg solid |
| Storage Temp. | −20°C |
| UNSPSC | 12352204 |